Anti-Inflammatory Effects of alpha-Galactosylceramide Analogs in Activated Microglia: Involvement of the p38 MAPK Signaling Pathway
DC Field | Value | Language |
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dc.contributor.author | 김용주 | - |
dc.date.accessioned | 2022-04-11T02:43:23Z | - |
dc.date.available | 2022-04-11T02:43:23Z | - |
dc.date.created | 2022-04-08 | - |
dc.date.issued | 2014-02 | - |
dc.identifier.issn | 19326203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/139970 | - |
dc.description.abstract | Microglial activation plays a pivotal role in the development and progression of neurodegenerative diseases. Thus, anti-inflammatory agents that control microglial activation can serve as potential therapeutic agents for neurodegenerative diseases. Here, we designed and synthesized alpha-galactosylceramide (alpha-GalCer) analogs to exert anti-inflammatory effects in activated microglia. We performed biological evaluations of 25 alpha-GalCer analogs and observed an interesting preliminary structure-activity relationship in their inhibitory influence on NO release and TNF-alpha production in LPS-stimulated BV2 microglial cells. After identification of 4d and 4e as hit compounds, we further investigated the underlying mechanism of their anti-inflammatory effects using RT-PCR analysis. We confirmed that 4d and 4e regulate the expression of iNOS, COX-2, IL-1 beta, and IL-6 at the mRNA level and the expression of TNF-alpha at the post-transcriptional level. In addition, both 4d and 4e inhibited LPS-induced DNA binding activities of NF-kappa B and AP-1 and phosphorylation of p38 MAPK without affecting other MAP kinases. When we examined the anti-inflammatory effect of a p38 MAPK-specific inhibitor, SB203580, on microglial activation, we observed an identical inhibitory pattern as that o | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.title | Anti-Inflammatory Effects of alpha-Galactosylceramide Analogs in Activated Microglia: Involvement of the p38 MAPK Signaling Pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 김용주 | - |
dc.identifier.doi | 10.1371/journal.pone.0087030 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.9, no.2 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 9 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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