Molecular characterization of wheat floret development-related F-box protein (TaF-box2): Possible involvement in regulation of Arabidopsis flowering
DC Field | Value | Language |
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dc.contributor.author | Kim, Jae Ho | - |
dc.contributor.author | Jung, Woo Joo | - |
dc.contributor.author | Kim, Moon Seok | - |
dc.contributor.author | Ko, Chan Seop | - |
dc.contributor.author | Yoon, Jin Seok | - |
dc.contributor.author | Hong, Min Jeong | - |
dc.contributor.author | Shin, Hyo Jeong | - |
dc.contributor.author | Seo, Yong Weon | - |
dc.date.accessioned | 2022-05-17T10:01:24Z | - |
dc.date.available | 2022-05-17T10:01:24Z | - |
dc.date.created | 2022-05-17 | - |
dc.date.issued | 2022-03 | - |
dc.identifier.issn | 0031-9317 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141114 | - |
dc.description.abstract | In wheat (Triticum aestivum L.), the floret development stage is an important step in determining grain yield per spike; however, the molecular mechanisms underlying floret development remain unclear. In this study, we elucidated the role of TaF-box2, a member of the F-box-containing E3 ubiquitin protein ligases, which is involved in floret development and anthesis of wheat. TaF-box2 was transiently expressed in the plasma membrane and cytoplasm of both tobacco and wheat. We also found that the SCFF-box2(Skp1-Cul1-Rbx1-TaF-box2) ubiquitin ligase complex mediated self-ubiquitination activity. Transgenic Arabidopsis plants that constitutively overexpressed TaF-box2 showed markedly greater hypocotyl and root length than wild-type plants, and produced early flowering phenotypes. Flowering-related genes were significantly upregulated in TaF-box2-overexpressing Arabidopsis plants. Further protein interaction analyses such as yeast two-hybrid, in vitro pull-down, and bimolecular fluorescence complementation assays confirmed that TaF-box2 physically interacted with TaCYCL1 (Triticum aestivum cyclin-L1-1). Ubiquitination and degradation assays demonstrated that TaCYCL1 was ubiquitinated by SCFF-box2 and degraded through the 26S proteasome complex. The physiological functions of the TaF-box2 protein remain unclear; however, we discuss several potential routes of involvement in various physiological mechanisms which counteract flowering in transgenic Arabidopsis plants. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | GENE | - |
dc.subject | DEGRADATION | - |
dc.subject | GROWTH | - |
dc.subject | TRANSITION | - |
dc.subject | EXPRESSION | - |
dc.subject | VERNALIZATION | - |
dc.subject | COMPLEXES | - |
dc.subject | REVEALS | - |
dc.subject | PATHWAY | - |
dc.subject | LEAF | - |
dc.title | Molecular characterization of wheat floret development-related F-box protein (TaF-box2): Possible involvement in regulation of Arabidopsis flowering | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seo, Yong Weon | - |
dc.identifier.doi | 10.1111/ppl.13677 | - |
dc.identifier.scopusid | 2-s2.0-85129197839 | - |
dc.identifier.wosid | 000787837300010 | - |
dc.identifier.bibliographicCitation | PHYSIOLOGIA PLANTARUM, v.174, no.2 | - |
dc.relation.isPartOf | PHYSIOLOGIA PLANTARUM | - |
dc.citation.title | PHYSIOLOGIA PLANTARUM | - |
dc.citation.volume | 174 | - |
dc.citation.number | 2 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | TRANSITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | VERNALIZATION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | REVEALS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | LEAF | - |
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