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Bio-isopropanol production in Corynebacterium glutamicum: Metabolic redesign of synthetic bypasses and two-stage fermentation with gas stripping

Authors
Ko, Young JinCha, JoyJeong, Wu-YoungLee, Myeong-EunCho, Byeong-HyeonNisha, BhardwajJeong, Hyun JinPark, Sung EunHan, Sung Ok
Issue Date
6월-2022
Publisher
ELSEVIER SCI LTD
Keywords
Isopropanol; Corynebacterium glutamicum; Synthetic metabolic bypass; Flux redistribution; Metabolic engineering
Citation
BIORESOURCE TECHNOLOGY, v.354
Indexed
SCIE
SCOPUS
Journal Title
BIORESOURCE TECHNOLOGY
Volume
354
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/141699
DOI
10.1016/j.biortech.2022.127171
ISSN
0960-8524
Abstract
Isopropanol is a commodity chemical widely used as a biofuel, fuel additive, rubbing alcohol and intermediate in various fields. Here, an engineered Corynebacterium glutamicum overproducing isopropanol was developed. To our knowledge, despite a representative industrial host to produce valuable chemicals, the high-level production of isopropanol in C. glutamicum has never been reported. First, the problem of the inability to produce isopropanol was solved by finding a key factor in its metabolism. The consolidation and modular optimization of synthetic bypasses including succinate and mevalonate bypasses enhanced isopropanol production. Flux redistribution of central metabolism significantly directed the carbon flux toward isopropanol biosynthesis. The final engineered strain produced 10.25 +/- 1.12 g/L isopropanol in two-stage fed-batch fermentation with an optimized gas stripping, which is the highest titer, yield and productivity in C. glutamicum. These strategies could be useful for the high-level production of isopropanol in C. glutamicum.
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