Novel Detection Method for Circulating EGFR Tumor DNA Using Gravitationally Condensed Gold Nanoparticles and Catalytic Walker DNA
DC Field | Value | Language |
---|---|---|
dc.contributor.author | You, Juneseok | - |
dc.contributor.author | Park, Chanho | - |
dc.contributor.author | Jang, Kuewhan | - |
dc.contributor.author | Park, Jinsung | - |
dc.contributor.author | Na, Sungsoo | - |
dc.date.accessioned | 2022-06-09T18:40:33Z | - |
dc.date.available | 2022-06-09T18:40:33Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-05 | - |
dc.identifier.issn | 1996-1944 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141761 | - |
dc.description.abstract | The detection of circulating tumor DNA is a major challenge in liquid biopsies for cancer. Conventionally, quantitative polymerase chain reactions or next-generation sequencing are used to detect circulating tumor DNA; however, these techniques require significant expertise, and are expensive. Owing to the increasing demand for a simple diagnostic method and constant monitoring of cancer, a cost-effective detection technique that can be conducted by non-experts is required. The aim of this study was to detect the circulating tumor DNA containing the epidermal growth factor receptor (EGFR) exon 19 deletion, which frequently occurs in lung cancer. By applying walker DNA to a catalytic hairpin assembly and using the differential dispersibility of gold nanoparticles, we detected EGFR exon 19 deletion mutant #2 DNA associated with lung cancer. Our sensing platform exhibited a limit of detection of 38.5 aM and a selectivity of 0.1% for EGFR exon 19 wild-type DNA. Moreover, we tested and compared EGFR exon 19 deletion mutants #1 and #3 to evaluate the effect of base pair mismatches on the performance of the said technique. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | STRAND DISPLACEMENT AMPLIFICATION | - |
dc.subject | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject | ROLLING CIRCLE AMPLIFICATION | - |
dc.subject | CELL-FREE DNA | - |
dc.subject | ELECTROCHEMICAL BIOSENSOR | - |
dc.subject | CANCER | - |
dc.subject | PLASMA | - |
dc.subject | PLATFORM | - |
dc.subject | BLOOD | - |
dc.subject | ASSAY | - |
dc.title | Novel Detection Method for Circulating EGFR Tumor DNA Using Gravitationally Condensed Gold Nanoparticles and Catalytic Walker DNA | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Na, Sungsoo | - |
dc.identifier.doi | 10.3390/ma15093301 | - |
dc.identifier.scopusid | 2-s2.0-85130632767 | - |
dc.identifier.wosid | 000794410200001 | - |
dc.identifier.bibliographicCitation | MATERIALS, v.15, no.9 | - |
dc.relation.isPartOf | MATERIALS | - |
dc.citation.title | MATERIALS | - |
dc.citation.volume | 15 | - |
dc.citation.number | 9 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Metallurgy & Metallurgical Engineering | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Metallurgy & Metallurgical Engineering | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | STRAND DISPLACEMENT AMPLIFICATION | - |
dc.subject.keywordPlus | MEDIATED ISOTHERMAL AMPLIFICATION | - |
dc.subject.keywordPlus | ROLLING CIRCLE AMPLIFICATION | - |
dc.subject.keywordPlus | CELL-FREE DNA | - |
dc.subject.keywordPlus | ELECTROCHEMICAL BIOSENSOR | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | PLASMA | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | BLOOD | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | circulating tumor DNA | - |
dc.subject.keywordAuthor | walker DNA | - |
dc.subject.keywordAuthor | gold nanoparticle | - |
dc.subject.keywordAuthor | UV-vis absorbance | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.