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Differential staining lowers the false positive detection in a novel volumetric measurement technique of microplastics

Authors
Tarafdar, AbhrajyotiChoi, Sang-HyunKwon, Jung-Hwan
Issue Date
15-6월-2022
Publisher
ELSEVIER
Keywords
Confocal microscopy; Z-stack CLSM; Microplastics volumetric measurement; Nile Red detection; Distinctive dyeing
Citation
JOURNAL OF HAZARDOUS MATERIALS, v.432
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF HAZARDOUS MATERIALS
Volume
432
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/141809
DOI
10.1016/j.jhazmat.2022.128755
ISSN
0304-3894
Abstract
A novel method for the volumetric detection of microplastics in various environmental (soil, water) and food (fish, meat) matrices was developed. The method is based on the Nile Red staining of microplastics while eliminating probable interference by other organic polymers such as lignin, chitin, cellulosic materials, and other organic substances using a mixture of three water-based dyes (Calcofluor White, Evans Blue, and 4,6-diamidino2-phenylindole [DAPI]). The excitation/emission 'sweet spot' was determined for water based blue dyes to detect them in a single channel for effective elimination of probable contaminations. Detection of microplastic particles using the Nile Red method was validated by comparing with traditional detection of microplastics via Fourier transform infrared spectroscopy (FTIR). Volumetric measurements of the microplastics present in environmental samples were made possible using Z-stack confocal microscopy images backed by threshold-based 3D segmentation. Regularly shaped microplastic materials were used to validate the volumetric measurement method. The proposed volumetric determination method will be very useful for screening microplastics in diverse media and improving the prevailing method using FTIR.
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College of Life Sciences and Biotechnology > Division of Environmental Science and Ecological Engineering > 1. Journal Articles

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