Double Ki-67 and synaptophysin labeling in pancreatic neuroendocrine tumor biopsies
DC Field | Value | Language |
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dc.contributor.author | Ahn, Bokyung | - |
dc.contributor.author | Jung, Jin Kying | - |
dc.contributor.author | Jung, HaeSung | - |
dc.contributor.author | Ryu, Yeon-Mi | - |
dc.contributor.author | Kim, Yeon Wook | - |
dc.contributor.author | Song, Tae Jun | - |
dc.contributor.author | Park, Do Hyun | - |
dc.contributor.author | Hwang, Dae Wook | - |
dc.contributor.author | Cho, HyungJun | - |
dc.contributor.author | Kim, Sang-Yeob | - |
dc.contributor.author | Hong, Seung-Mo | - |
dc.date.accessioned | 2022-06-10T14:40:45Z | - |
dc.date.available | 2022-06-10T14:40:45Z | - |
dc.date.created | 2022-06-09 | - |
dc.date.issued | 2022-04 | - |
dc.identifier.issn | 1424-3903 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/141862 | - |
dc.description.abstract | Background: Pancreatic neuroendocrine tumors (PanNETs) are frequently detected on endoscopic ultrasound-guided fine-needle aspiration biopsy (EUS-FNAB) specimens. The conventional methods for evaluating the Ki-67 labeling index (Ki67LI) in EUS-FNAB specimens are laborious, and their results are difficult to interpret. More practical and easy methods for evaluating the Ki67LI in PanNETs from EUSFNAB specimens is increasing in need. Methods: We used double Ki-67 and synaptophysin (double Ki-Syn) antibody cocktail; Ki67LI, total Ki-67 positive cells, and total tumor cells were counted and compared with those detected on conventional single Ki-67 immunostaining (single Ki-67) of 96 PanNETs [Grade 1 (G1), 68 cases (71%); G2, 26 (27%); G3, 2 (2%)] from EUS-FNAB specimens. Results: The tumor grading between double Ki-Syn and single Ki-67 immunolabeling was highly concordant (correlation, 0.95; Fisher's exact test, P < 0.001). Seven EUS-FNAB specimens (7%) had discrepant results, of which 2 were removed through surgical resection and showed the same tumor grade as that detected on double Ki-Syn immunolabeling. Fifty-four specimens (56%) had higher Ki-67 positive tumor cell counts on single Ki-67 immunolabeling. Sixty-two specimens (65%) had higher total tumor cell counts on double Ki-Syn immunolabeling. The number of specimens with less than 500 total counted tumor cells were significantly reduced when double Ki-Syn immunolabeling was applied [P = 0.046; single Ki-67, 17 specimens (18%); double Ki-Syn, 9 specimens (9%)]. Conclusion: Double Ki-Syn immunolabeling enables the accurate counting of the number of proliferating tumor cells without including inflammatory and contaminant epithelial cells compared with single Ki-67 immunolabeling in PanNETs from EUS-FNAB specimens. (C) 2022 IAP and EPC. Published by Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | FINE-NEEDLE-ASPIRATION | - |
dc.subject | INDEX | - |
dc.title | Double Ki-67 and synaptophysin labeling in pancreatic neuroendocrine tumor biopsies | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, HyungJun | - |
dc.identifier.doi | 10.1016/j.pan.2022.03.005 | - |
dc.identifier.scopusid | 2-s2.0-85126303637 | - |
dc.identifier.wosid | 000792208400013 | - |
dc.identifier.bibliographicCitation | PANCREATOLOGY, v.22, no.3, pp.427 - 434 | - |
dc.relation.isPartOf | PANCREATOLOGY | - |
dc.citation.title | PANCREATOLOGY | - |
dc.citation.volume | 22 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 427 | - |
dc.citation.endPage | 434 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Gastroenterology & Hepatology | - |
dc.relation.journalWebOfScienceCategory | Gastroenterology & Hepatology | - |
dc.subject.keywordPlus | FINE-NEEDLE-ASPIRATION | - |
dc.subject.keywordPlus | INDEX | - |
dc.subject.keywordAuthor | Pancreas | - |
dc.subject.keywordAuthor | Neuroendocrine tumor | - |
dc.subject.keywordAuthor | Grading | - |
dc.subject.keywordAuthor | Ki-67 | - |
dc.subject.keywordAuthor | Synaptophysin | - |
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