Biosensor-Linked Immunosorbent Assay for the Quantification of Methionine Oxidation in Target Proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hae Min | - |
dc.contributor.author | Choi, Dong Wook | - |
dc.contributor.author | Kim, Seahyun | - |
dc.contributor.author | Lee, Aro | - |
dc.contributor.author | Kim, Minseo | - |
dc.contributor.author | Roh, Yeon Jin | - |
dc.contributor.author | Jo, Young Ho | - |
dc.contributor.author | Cho, Hwa Yeon | - |
dc.contributor.author | Lee, Ho Jae | - |
dc.contributor.author | Lee, Seung-Rock | - |
dc.contributor.author | Tarrago, Lionel | - |
dc.contributor.author | Gladyshev, Vadim N. | - |
dc.contributor.author | Kim, Ji Hyung | - |
dc.contributor.author | Lee, Byung Cheon | - |
dc.date.accessioned | 2022-06-12T11:40:27Z | - |
dc.date.available | 2022-06-12T11:40:27Z | - |
dc.date.created | 2022-06-10 | - |
dc.date.issued | 2022-01 | - |
dc.identifier.issn | 2379-3694 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/142131 | - |
dc.description.abstract | Methionine oxidation is involved in regulating the protein activity and often leads to protein malfunction. However, tools for quantitative analyses of protein-specific methionine oxidation are currently unavailable. In this work, we developed a biological sensor that quantifies oxidized methionine in the form of methionine-R-sulfoxide in target proteins. The biosensor "tpMetROG" consists of methionine sulfoxide reductase B (MsrB), circularly permuted yellow fluorescent protein (cpYFP), thioredoxin, and protein G. Protein G binds to the constant region of antibodies against target proteins, specifically capturing them. Then, MsrB reduces the oxidized methionine in these proteins, leading to cpYFP fluorescence changes. We assessed this biosensor for quantitative analysis of methionine-R-sulfoxide in various proteins, such as calmodulin, IDLO, LegP, Sacde, and actin. We further developed an immunosorbent assay using the biosensor to quantify methionine oxidation in specific proteins such as calmodulin in animal tissues. The biosensor-linked immunosorbent assay proves to be an indispensable tool for detecting methionine oxidation in a protein-specific manner. This is a versatile tool for studying the redox biology of methionine oxidation in proteins. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CALMODULIN | - |
dc.subject | SULFOXIDE | - |
dc.subject | ACTIVATION | - |
dc.subject | STRESS | - |
dc.subject | IMPACT | - |
dc.subject | MSRB1 | - |
dc.title | Biosensor-Linked Immunosorbent Assay for the Quantification of Methionine Oxidation in Target Proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Ji Hyung | - |
dc.identifier.doi | 10.1021/acssensors.1c01819 | - |
dc.identifier.scopusid | 2-s2.0-85122598212 | - |
dc.identifier.wosid | 000736550000001 | - |
dc.identifier.bibliographicCitation | ACS SENSORS, v.7, no.1, pp.131 - 141 | - |
dc.relation.isPartOf | ACS SENSORS | - |
dc.citation.title | ACS SENSORS | - |
dc.citation.volume | 7 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 131 | - |
dc.citation.endPage | 141 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | CALMODULIN | - |
dc.subject.keywordPlus | SULFOXIDE | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | MSRB1 | - |
dc.subject.keywordAuthor | methionine oxidation | - |
dc.subject.keywordAuthor | biosensor | - |
dc.subject.keywordAuthor | methionine sulfoxide reductase B | - |
dc.subject.keywordAuthor | immunosorbent assay | - |
dc.subject.keywordAuthor | redox | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.