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Scalable Functionalization of Polyaniline-Grafted rGO Field-Effect Transistors for a Highly Sensitive Enzymatic Acetylcholine Biosensoropen access

Authors
Park, DongsungLee, DongtakKim, Hye JinYoon, Dae SungHwang, Kyo Seon
Issue Date
5월-2022
Publisher
MDPI
Keywords
reduced graphene oxide; field-effect transistor (FET); polyaniline; pH-sensing; acetylcholine; acetylcholinesterase; drug screening
Citation
BIOSENSORS-BASEL, v.12, no.5
Indexed
SCIE
SCOPUS
Journal Title
BIOSENSORS-BASEL
Volume
12
Number
5
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/142277
DOI
10.3390/bios12050279
ISSN
2079-6374
Abstract
For decades, acetylcholine (Ach) has been considered a critical biomarker for several degenerative brain diseases, including Alzheimer's, Parkinson's disease, Huntington's disease, and schizophrenia. Here, we propose a wafer-scale fabrication of polyaniline (PAni)-grafted graphene-based field-effect transistors (PGFET) and their biosensing applications for highly sensitive and reliable real-time monitoring of Ach in flow configuration. The grafted PAni provides suitable electrostatic binding sites for enzyme immobilization and enhances the pH sensitivity (2.68%/pH), compared to that of bare graphene-FET (1.81%/pH) for a pH range of 3-9 without any pH-hysteresis. We further evaluated the PGFET's sensing performance for Ach detection with a limit of detection at the nanomolar level and significantly improved sensitivity (similar to 103%) in the concentration range of 108 nM to 2 mM. Moreover, the PGFET exhibits excellent selectivity against various interferences, including glucose, ascorbic acid, and neurotransmitters dopamine and serotonin. Finally, we investigated the effects of an inhibitor (rivastigmine) on the AchE activity of the PGFET. From the results, we demonstrated that the PGFET has great potential as a real-time drug-screening platform by monitoring the inhibitory effects on enzymatic activity.
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