Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Enriching intracellular macrolides in Escherichia coli improved the sensitivity of bioluminescent sensing systems

Authors
Kim, Duck GyunKim, MinsunOh, Min-Kyu
Issue Date
1-11월-2022
Publisher
ELSEVIER
Keywords
Bioluminescent sensing system; Efflux pump; Macrolide sensing system; Erythromycin resistance repressor protein
Citation
TALANTA, v.249
Indexed
SCIE
SCOPUS
Journal Title
TALANTA
Volume
249
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/142709
DOI
10.1016/j.talanta.2022.123626
ISSN
0039-9140
Abstract
A repressor protein MphR and an enhanced green fluorescent protein (eGFP) were used to construct a bioluminescent sensing system for macrolide analysis in Escherichia coli host cells. We deleted TolC, an efflux pump for macrolides in E. coli, to promote the intracellular accumulation of macrolides. The binding constant (K1/2) of the sensing system constructed in an E. coli strain was decreased up to 33-fold with deleted TolC, and its sensitivity to the macrolides erythromycin, azithromycin, roxithromycin, and pikromycin was increased. The limit of detection of the bioluminescent sensing system for serum azithromycin was 4.1 nM. The ability to detect serum azithromycin concentrations was confirmed by analyzing photographs using ImageJ software. We also developed a novel sensing system for the immune suppressor FK506, another macrolide that is frequently prescribed. Deleting TolC also significantly improved the sensitivity of this sensing system. Bioluminescent sensing systems constructed in TolC mutants were sensitive to various macrolides, indicating their potential for clinical application with hand-held devices.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Engineering > Department of Chemical and Biological Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Altmetrics

Total Views & Downloads

BROWSE