Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose-stimulated vascular endothelial and smooth muscle cells
DC Field | Value | Language |
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dc.contributor.author | Han, A. Young | - |
dc.contributor.author | Ha, Su Min | - |
dc.contributor.author | Shin, You Kyoung | - |
dc.contributor.author | Seol, Geun Hee | - |
dc.date.accessioned | 2022-08-10T23:40:31Z | - |
dc.date.available | 2022-08-10T23:40:31Z | - |
dc.date.created | 2022-08-10 | - |
dc.date.issued | 2022-06-22 | - |
dc.identifier.issn | 2662-7671 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/142791 | - |
dc.description.abstract | Background Ginsenoside Rg-1 (Rg-1), a triterpenoid saponin abundantly present in Panax ginseng, is a type of naturally occurring steroid with known anti-diabetic and anti-inflammatory effects. In this study, we sought to confirm the effects and mechanisms of action of Rg-1 on store-operated Ca2+ entry (SOCE) in human vascular endothelial cell line (EA) and murine aortic vascular smooth muscle cell line (MOVAS) cells exposed to high glucose. Methods Cytosolic Ca2+ concentrations in EA and MOVAS cells were measured by monitoring fluorescence of the ratiometric Ca2+-indicator, Fura-2 AM. Results High glucose significantly increased Ca2+ influx by abnormally activating SOCE in EA and MOVAS cells. Notably, this high glucose-induced increase in SOCE was restored to normal levels in EA and MOVAS cells by Rg-1. Moreover, Rg-1 induced reductions in SOCE in cells exposed to high glucose were significantly inhibited by the plasma membrane Ca2+ ATPase (PMCA) blocker lanthanum, the Na+/K+-ATPase blocker ouabain, or the Na+/Ca2+ exchanger (NCX) blockers Ni2+ and KB-R7943. These observations suggest that the mechanism of action of Rg-1 inhibition of SOCE involves PMCA and Na+/K+-ATPase, and an increase in Ca2+ efflux via NCXs in both EA and MOVAS cells exposed to high glucose. Conclusions These findings indicate that Rg-1 may protect vascular endothelial and smooth muscle cells from Ca2+ increases following exposure to hyperglycemic conditions. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | PLASMA-MEMBRANE CA2+-ATPASE | - |
dc.subject | CALCIUM-ENTRY | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | EXPRESSION | - |
dc.subject | RG1 | - |
dc.subject | MITOCHONDRIAL | - |
dc.subject | CALCINEURIN | - |
dc.subject | APOPTOSIS | - |
dc.subject | INJURY | - |
dc.subject | INFLUX | - |
dc.title | Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose-stimulated vascular endothelial and smooth muscle cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Seol, Geun Hee | - |
dc.identifier.doi | 10.1186/s12906-022-03647-5 | - |
dc.identifier.scopusid | 2-s2.0-85132419315 | - |
dc.identifier.wosid | 000814746300002 | - |
dc.identifier.bibliographicCitation | BMC COMPLEMENTARY MEDICINE AND THERAPIES, v.22, no.1 | - |
dc.relation.isPartOf | BMC COMPLEMENTARY MEDICINE AND THERAPIES | - |
dc.citation.title | BMC COMPLEMENTARY MEDICINE AND THERAPIES | - |
dc.citation.volume | 22 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.subject.keywordPlus | PLASMA-MEMBRANE CA2+-ATPASE | - |
dc.subject.keywordPlus | CALCIUM-ENTRY | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | RG1 | - |
dc.subject.keywordPlus | MITOCHONDRIAL | - |
dc.subject.keywordPlus | CALCINEURIN | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | INFLUX | - |
dc.subject.keywordAuthor | Ginsenoside Rg-1 | - |
dc.subject.keywordAuthor | Store-operated Ca2+ entry | - |
dc.subject.keywordAuthor | High glucose | - |
dc.subject.keywordAuthor | Vascular endothelial cells | - |
dc.subject.keywordAuthor | Vascular smooth muscle cells | - |
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