Recapitulated Crosstalk between Cerebral Metastatic Lung Cancer Cells and Brain Perivascular Tumor Microenvironment in a Microfluidic Co-Culture Chip
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunho | - |
dc.contributor.author | Sa, Jason K. | - |
dc.contributor.author | Kim, Jaehoon | - |
dc.contributor.author | Cho, Hee Jin | - |
dc.contributor.author | Oh, Hyun Jeong | - |
dc.contributor.author | Choi, Dong-Hee | - |
dc.contributor.author | Kong, Seok-Hyeon | - |
dc.contributor.author | Jeong, Da Eun | - |
dc.contributor.author | Nam, Do-Hyun | - |
dc.contributor.author | Lee, Hakho | - |
dc.contributor.author | Lee, Hye Won | - |
dc.contributor.author | Chung, Seok | - |
dc.date.accessioned | 2022-08-15T04:40:54Z | - |
dc.date.available | 2022-08-15T04:40:54Z | - |
dc.date.created | 2022-08-12 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 2198-3844 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/143232 | - |
dc.description.abstract | Non-small cell lung carcinoma (NSCLC), which affects the brain, is fatal and resistant to anti-cancer therapies. Despite innate, distinct characteristics of the brain from other organs, the underlying delicate crosstalk between brain metastatic NSCLC (BM-NSCLC) cells and brain tumor microenvironment (bTME) associated with tumor evolution remains elusive. Here, a novel 3D microfluidic tri-culture platform is proposed for recapitulating positive feedback from BM-NSCLC and astrocytes and brain-specific endothelial cells, two major players in bTME. Advanced imaging and quantitative functional assessment of the 3D tri-culture model enable real-time live imaging of cell viability and separate analyses of genomic/molecular/secretome from each subset. Susceptibility of multiple patient-derived BM-NSCLCs to representative targeted agents is altered and secretion of serpin E1, interleukin-8, and secreted phosphoprotein 1, which are associated with tumor aggressiveness and poor clinical outcome, is increased in tri-culture. Notably, multiple signaling pathways involved in inflammatory responses, nuclear factor kappa-light-chain-enhancer of activated B cells, and cancer metastasis are activated in BM-NSCLC through interaction with two bTME cell types. This novel platform offers a tool to elucidate potential molecular targets and for effective anti-cancer therapy targeting the crosstalk between metastatic cancer cells and adjacent components of bTME. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | CHEMOTHERAPY | - |
dc.subject | CULTURE | - |
dc.subject | GENES | - |
dc.title | Recapitulated Crosstalk between Cerebral Metastatic Lung Cancer Cells and Brain Perivascular Tumor Microenvironment in a Microfluidic Co-Culture Chip | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Sa, Jason K. | - |
dc.identifier.doi | 10.1002/advs.202201785 | - |
dc.identifier.scopusid | 2-s2.0-85131183968 | - |
dc.identifier.wosid | 000805377700001 | - |
dc.identifier.bibliographicCitation | ADVANCED SCIENCE, v.9, no.22 | - |
dc.relation.isPartOf | ADVANCED SCIENCE | - |
dc.citation.title | ADVANCED SCIENCE | - |
dc.citation.volume | 9 | - |
dc.citation.number | 22 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | CHEMOTHERAPY | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordAuthor | brain perivascular tumor microenvironment | - |
dc.subject.keywordAuthor | cerebral metastatic lung cancer cells | - |
dc.subject.keywordAuthor | microfluidic co-culture chip | - |
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