Aurantiacibacter sediminis sp. nov., a marine bacterium isolated from a tidal flat

Abstract

A yellow- coloured bacterium, designated as strain JGD- 13T, was isolated from a tidal flat in the Republic of Korea. Cells were Gram- stain- negative, aerobic, non- flagellated and rod- shaped. Growth was observed at 4???42 ??C (optimum, 30 ??C), at pH 6.0??? 12.0 (pH 7.0???8.0) and at 1???7 % (w/v) NaCl concentration (3 %). The 16S rRNA gene sequence analysis indicated that strain JGD-13T was closely related to Aurantiacibacter gangjinensis K7-2T with a sequence similarity of 98.2 %, followed by Aurantiacibacter aquimixticola JSSK-14T (98.1 %), Aurantiacibacter atlanticus s21-N3T (97.6 %), Aurantiacibacter zhengii V18T (97.6 %) and Aurantiacibacter luteus KA37T (97.5 %). The average nucleotide identity and digital DNA???DNA hybridization values with related strains were 70.3???76.2 % and 18.5???20.3 %. The genomic DNA G+C content was 60.2 mol%. Phylogenetic analysis using the maximum- likelihood method showed that strain JGD- 13T formed a clade with A. aquimixticola JSSK-14T and A. gangjinensis K7-2T. The major fatty acids were summed feature 8 (39.7 %) and C17:1 ??6c (14.4 %). The predominant respiratory quinone was ubiquinone- 10. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one sphingoglycolipid and three unidentified lipids. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain JGD13T represents a novel species within the genus Aurantiacibacter, for which the name Aurantiacibacter sediminis JGD-13Tsp. nov. is proposed. The type strain is JGD- 13T (=KCTC 72892T=KACC 21676T=JCM 33995T).