Protective Effect of Ulinastatin on Cognitive Function After Hypoxia
- Authors
- Kim, Young Sung; Sohn, Sung-Hwa; Min, Too Jae
- Issue Date
- 1월-2022
- Publisher
- HUMANA PRESS INC
- Keywords
- Ulinastatin; GABA(A)R; Hypoxia; Postoperative cognitive dysfunction
- Citation
- NEUROMOLECULAR MEDICINE
- Indexed
- SCIE
SCOPUS
- Journal Title
- NEUROMOLECULAR MEDICINE
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/143604
- DOI
- 10.1007/s12017-022-08721-2
- ISSN
- 1535-1084
- Abstract
- Ulinastatin (UTI) has neuroprotective properties. Neurologic insults, including hypoxia and use of anesthetic agents, cause postoperative cognitive dysfunction and alter gamma-aminobutyric acid (GABA) function. This study aimed to assess whether UTI could preserve learning and memory using a zebrafish hypoxic behavior model and biomarkers. Zebrafish (6-8 months of age and 2.5-3.5 cm long) were divided into eight groups as follows: phosphate-buffered saline (PBS) control, hypoxia + PBS, UTI (10,000, 50,000, and 100,000 units/kg), and hypoxia with UTI (10,000, 50,000, and 100,000 units/kg) groups. The endpoints of the T-maze experiment included total time, distance moved, and frequency in target or opposite compartment. We also measured the degree of brain infarction using 2,3,5-triphenyltetrazolium chloride staining, assessed SA-beta-galactosidase activity, and examined GABA(A) receptor expression using real-time polymerase chain reaction. In a dose-dependent manner, UTI affected learning and memory in zebrafish. Despite hypoxia, 100,000 units/kg of UTI preserved preference (time and distance) for the target compartment. More than 50,000 units/kg of UTI also showed reduced hypoxia-induced brain infarction, decreased SA-beta-galactosidase levels, and upregulated GABA(A) receptors. This study demonstrated that the location of the GABA(A) receptor is affected by hypoxia or UTI.
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Collections - College of Medicine > Department of Medical Science > 1. Journal Articles
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