Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Wonjun | - |
dc.contributor.author | Kang, Munkyu | - |
dc.contributor.author | Hong, Jin Hee | - |
dc.contributor.author | Katz, Ori | - |
dc.contributor.author | Lee, Byunghak | - |
dc.contributor.author | Kim, Guang Hoon | - |
dc.contributor.author | Choi, Youngwoon | - |
dc.contributor.author | Choi, Wonshik | - |
dc.date.accessioned | 2022-09-23T22:41:18Z | - |
dc.date.available | 2022-09-23T22:41:18Z | - |
dc.date.created | 2022-09-23 | - |
dc.date.issued | 2022-08-02 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/143803 | - |
dc.description.abstract | Lensless fibre endoscopes are minimally invasive, but are often rigid and require calibration and fluorescence labelling. Here, the authors present a flexible endoscope based on a bare fibre bundle and a lensless Fourier holographic imaging configuration to detect weak reflections from unstained biological tissues. Ultrathin lensless fibre endoscopes offer minimally invasive investigation, but they mostly operate as a rigid type due to the need for prior calibration of a fibre probe. Furthermore, most implementations work in fluorescence mode rather than label-free imaging mode, making them unsuitable for general medical diagnosis. Herein, we report a fully flexible ultrathin fibre endoscope taking 3D holographic images of unstained tissues with 0.85-mu m spatial resolution. Using a bare fibre bundle as thin as 200-mu m diameter, we design a lensless Fourier holographic imaging configuration to selectively detect weak reflections from biological tissues, a critical step for label-free endoscopic reflectance imaging. A unique algorithm is developed for calibration-free holographic image reconstruction, allowing us to image through a narrow and curved passage regardless of fibre bending. We demonstrate endoscopic reflectance imaging of unstained rat intestine tissues that are completely invisible to conventional endoscopes. The proposed endoscope will expedite a more accurate and earlier diagnosis than before with minimal complications. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.subject | 2-PHOTON MICROSCOPE | - |
dc.subject | FIBER BUNDLE | - |
dc.subject | ENDOMICROSCOPY | - |
dc.subject | COMPENSATION | - |
dc.subject | DEEP | - |
dc.title | Flexible-type ultrathin holographic endoscope for microscopic imaging of unstained biological tissues | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Wonshik | - |
dc.identifier.doi | 10.1038/s41467-022-32114-5 | - |
dc.identifier.scopusid | 2-s2.0-85135267529 | - |
dc.identifier.wosid | 000836703600013 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.13, no.1 | - |
dc.relation.isPartOf | NATURE COMMUNICATIONS | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | 2-PHOTON MICROSCOPE | - |
dc.subject.keywordPlus | FIBER BUNDLE | - |
dc.subject.keywordPlus | ENDOMICROSCOPY | - |
dc.subject.keywordPlus | COMPENSATION | - |
dc.subject.keywordPlus | DEEP | - |
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