Protective effects of pentoxifylline on T-cell viability under inflammatory conditions
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Sung-Joon | - |
dc.contributor.author | Choi, Sung-Hyuk | - |
dc.contributor.author | Cho, Young-Duck | - |
dc.contributor.author | Kim, Jung-Youn | - |
dc.contributor.author | Cho, Han-Jin | - |
dc.contributor.author | Kim, Kyung-Hwan | - |
dc.contributor.author | Kim, Won-Young | - |
dc.date.accessioned | 2022-09-24T04:40:49Z | - |
dc.date.available | 2022-09-24T04:40:49Z | - |
dc.date.created | 2022-09-23 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 1721-727X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/143832 | - |
dc.description.abstract | Introduction: Pentoxifylline (PTX) reduces the levels of pro-inflammatory cytokines; however, its effects on immune system is not well understood. The aim of this study was to investigate the effect of PTX on T cells under inflammatory conditions in co-culture with THP-1-derived macrophages. Methods: Toll-like receptor 4 (TLR4) and macrophage migration inhibitory factor (MIF) levels were measured after addition of PTX to lipopolysaccharide (LPS)-stimulated differentiated THP-1 cells. T cell viability and MIF levels were measured after PTX was added to prostaglandin E-2 (PGE(2))-stimulated Jurkat T-cell leukemia line. Co-culture was conducted to determine the effect of LPS-stimulated differentiated THP-1 cells that are affected by PTX on Jurkat cells. To prevent the direct effects of LPS and PTX on Jurkat cells, LPS and PTX were washed from THP-1 cells before co-culture. T cell viability and interleukin-2 (IL-2) levels were determined in Jurkat cells. Results: Increase in the MIF concentration and TLR4 expression level in differentiated THP-1 cells stimulated with LPS were reversed after PTX addition. However, PTX did not improve T cell viability in PGE(2)-stimulated Jurkat cells. Co-culturing Jurkat cell and LPS-stimulated differentiated THP-1 cells resulted in a decreased viability of T cells. The addition of PTX restored T cell viability to normal control levels and IL-2 expression level in Jurkat cells. Conclusion: LPS-stimulated THP-1-derived macrophages reduced the T cell viability under inflammation. However, PTX restored T cells viability and IL-2 back to normal levels. Therefore, the immunomodulatory action of PTX may be mediated by macrophage-T cell interactions. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.subject | MIGRATION INHIBITORY FACTOR | - |
dc.subject | DENDRITIC CELLS | - |
dc.subject | SEVERE SEPSIS | - |
dc.subject | INTERLEUKIN-2 | - |
dc.subject | INCREASES | - |
dc.subject | MIF | - |
dc.title | Protective effects of pentoxifylline on T-cell viability under inflammatory conditions | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Sung-Hyuk | - |
dc.identifier.doi | 10.1177/1721727X221120753 | - |
dc.identifier.scopusid | 2-s2.0-85136973217 | - |
dc.identifier.wosid | 000846840200001 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF INFLAMMATION, v.20 | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF INFLAMMATION | - |
dc.citation.title | EUROPEAN JOURNAL OF INFLAMMATION | - |
dc.citation.volume | 20 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | INCREASES | - |
dc.subject.keywordPlus | INTERLEUKIN-2 | - |
dc.subject.keywordPlus | MIF | - |
dc.subject.keywordPlus | MIGRATION INHIBITORY FACTOR | - |
dc.subject.keywordPlus | SEVERE SEPSIS | - |
dc.subject.keywordAuthor | Trauma | - |
dc.subject.keywordAuthor | interleukin 2 | - |
dc.subject.keywordAuthor | lymphocyte | - |
dc.subject.keywordAuthor | pentoxifylline | - |
dc.subject.keywordAuthor | toll-like receptor | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.