Detailed Information

Cited 0 time in webofscience Cited 0 time in scopus
Metadata Downloads

Isolation and Characterization of Urinary Extracellular Vesicles from Healthy Donors and Patients with Castration-Resistant Prostate Canceropen access

Authors
Lee, HaneulKang, Su JinLee, JiminPark, Kyong HwaRhee, Won Jong
Issue Date
7월-2022
Publisher
MDPI
Keywords
liquid biopsy; extracellular vesicle; prognosis; urine; castration-resistant prostate cancer
Citation
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.23, no.13
Indexed
SCIE
SCOPUS
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume
23
Number
13
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/143876
DOI
10.3390/ijms23137134
ISSN
1661-6596
Abstract
Prostate cancer (PCa) is the most commonly diagnosed malignancy among men in developed countries. The five-year survival rate for men diagnosed with early-stage PCa is approximately 100%, while it is less than 30% for castration-resistant PCa (CRPC). Currently, the detection of prostate-specific antigens as biomarkers for the prognosis of CRPC is criticized because of its low accuracy, high invasiveness, and high false-positive rate. Therefore, it is important to identify new biomarkers for prediction of CRPC progression. Extracellular vesicles (EVs) derived from tumors have been highlighted as potential markers for cancer diagnosis and prognosis. Specifically, urinary EVs directly reflect changes in the pathophysiological conditions of the urogenital system because it is exposed to prostatic secretions. Thus, detecting biomarkers in urinary EVs provides a promising approach for performing an accurate and non-invasive liquid biopsy for CPRC. In this study, we effectively isolated urinary EVs with low protein impurities using size-exclusion chromatography combined with ultrafiltration. After EV isolation and characterization, we evaluated the miRNAs in urinary EVs from healthy donors and patients with CRPC. The results indicated that miRNAs (miR-21-5p, miR-574-3p, and miR-6880-5p) could be used as potential biomarkers for the prognosis of CRPC. This analysis of urinary EVs contributes to the fast and convenient prognosis of diseases, including CRPC, in the clinical setting.
Files in This Item
There are no files associated with this item.
Appears in
Collections
Graduate School > Department of Biomedical Sciences > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Kyong Hwa photo

Park, Kyong Hwa
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE