Efficacy Evaluation of SDF-1?-Based Polypeptides in an Acute Myocardial Infarction Model Using Structure-Based Drug Design
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Kang-Gon | - |
dc.contributor.author | Santos, Ana Rita M. P. | - |
dc.contributor.author | Kang, Yong Guk | - |
dc.contributor.author | Chae, Yun Jin | - |
dc.contributor.author | Shah, Masaud | - |
dc.contributor.author | Pirzada, Rameez Hassan | - |
dc.contributor.author | Song, Myeongjin | - |
dc.contributor.author | Kim, Jongseong | - |
dc.contributor.author | Choi, Sangdun | - |
dc.contributor.author | Park, Yongdoo | - |
dc.date.accessioned | 2022-11-15T13:40:51Z | - |
dc.date.available | 2022-11-15T13:40:51Z | - |
dc.date.created | 2022-11-15 | - |
dc.date.issued | 2022-10-10 | - |
dc.identifier.issn | 2373-9878 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/145482 | - |
dc.description.abstract | Stromal cell-derived factor-1 alpha (SDF-1 alpha, CXCL12) mediates the migration of circulating cells to desired sites for tissue development, homeostasis, and regeneration and can be used to promote cardiac regeneration by recruiting stem cells. However, the use of SDF-1 alpha in the injured heart necessitates not only higher binding affinity to its receptor, CXCR4+, but also better robustness against enzymatic degradation than other SDF-1 isoforms. Here, we conduct a screening of SDF-1 alpha analog peptides that were designed by structure-based drug design (SBDD), a type of computer-aided drug design (CADD). We have developed in vitro and in vivo methods that enable us to estimate the effect of peptides on the migration of human mesenchymal stem cells (hMSCs) and cardiac regeneration in acute myocardial infarction (AMI)-induced animals, respectively. We demonstrate that one type of SDF-1 alpha analog peptide, SDP-4, among the four analog peptides preselected by SBDD, is more potent than native SDF-1 alpha for cardiac regeneration in myocardial infarction. It is interesting to note that the migratory effects of SDP-4 determined by a wound healing assay, a Transwell assay, and a 2D migration assay are comparable to those of SDF-1 alpha. These results suggest that in vivo, as well as in vitro, screening of peptides developed by SBDD is a quintessential process to the development of a novel therapeutic compound for cardiac regeneration. Our finding also has an implication that the SDP-4 peptide is an excellent candidate for use in the regeneration of an AMI heart. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.subject | CELL-DERIVED FACTOR-1-ALPHA | - |
dc.subject | FACTOR-I | - |
dc.subject | PEPTIDE | - |
dc.subject | REGENERATION | - |
dc.subject | DISSOCIATION | - |
dc.subject | SDF-1 | - |
dc.title | Efficacy Evaluation of SDF-1?-Based Polypeptides in an Acute Myocardial Infarction Model Using Structure-Based Drug Design | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Yongdoo | - |
dc.identifier.doi | 10.1021/acsbiomaterials.2c00766Sci | - |
dc.identifier.scopusid | 2-s2.0-85139437034 | - |
dc.identifier.wosid | 000868138300001 | - |
dc.identifier.bibliographicCitation | ACS BIOMATERIALS SCIENCE & ENGINEERING, v.8, no.10, pp.4486 - 4496 | - |
dc.relation.isPartOf | ACS BIOMATERIALS SCIENCE & ENGINEERING | - |
dc.citation.title | ACS BIOMATERIALS SCIENCE & ENGINEERING | - |
dc.citation.volume | 8 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 4486 | - |
dc.citation.endPage | 4496 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | CELL-DERIVED FACTOR-1-ALPHA | - |
dc.subject.keywordPlus | FACTOR-I | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | DISSOCIATION | - |
dc.subject.keywordPlus | SDF-1 | - |
dc.subject.keywordAuthor | stromal cell-derived factor-1 alpha (SDF-1?) | - |
dc.subject.keywordAuthor | acute myocardial infarction (AMI) | - |
dc.subject.keywordAuthor | structure-based drug design (SBDD) | - |
dc.subject.keywordAuthor | computer-aided drug design (CADD) | - |
dc.subject.keywordAuthor | hyaluronic acid-based hydrogel (HA gels) | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.