Exposure to airborne particulate matter induces renal tubular cell injury in vitro: The role of vitamin D signaling and renin-angiotensin system
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Eungu | - |
dc.contributor.author | Yim, Hyung Eun | - |
dc.contributor.author | Nam, Yoon Jeong | - |
dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Kim, Joo-Ae | - |
dc.contributor.author | Lee, Ju-Han | - |
dc.contributor.author | Son, Min Hwa | - |
dc.contributor.author | Yoo, Kee Hwan | - |
dc.date.accessioned | 2022-11-16T01:41:00Z | - |
dc.date.available | 2022-11-16T01:41:00Z | - |
dc.date.created | 2022-11-15 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 2405-8440 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/145543 | - |
dc.description.abstract | Background: Exposure to air pollution can interfere with the vitamin D endocrine system. This study investigated the effects of airborne particulate matter (PM) on renal tubular cell injury in vitro and explored the underlying mechanisms.Methods: HK-2 human renal proximal tubule cells were treated with PM with or without 1,25(OH)2D3 analog, 19-Nor-1,25(OH)2D2 (paricalcitol, 10 nM) for 48 hours. The dose-and time -dependent cytotoxicity of PM with or without paricalcitol was determined via cell counting kit-8 assay. Cellular oxidative stress was assessed using commercially available enzyme-linked immunosorbent assay kits. The protein expression of vitamin D receptor (VDR), cytochrome P450(CYP)27B1, CYP24A1, renin, angiotensin converting enzyme (ACE), angiotensin II type 1 receptor (AT1), nuclear factor erythroid 2-related factor 2 (Nrf2), nuclear factor-kB (NF-kB), tumor necrosis factor (TNF)-alpha, and interleukin (IL)-6 was determined.Results: PM exposure decreased HK-2 cell viability in a dose-and time-dependent manner. The activities of superoxide dismutase and malondialdehyde in HK-2 cells increased significantly in the group exposed to PM. PM exposure decreased VDR and Nrf2, while increasing CYP27B1, renin, ACE, AT1, NF-kB, TNF-alpha, and IL-6. The expression of VDR, CYP27B1, renin, ACE, AT1, and TNF-alpha was reversed by paricalcitol treatment. Paricalcitol also restored the cell viability of PM-exposed HK-2 cells.Conclusion: Our findings indicate that exposure to PM induces renal proximal tubular cell injury, concomitant with alteration of vitamin D endocrine system and renin angiotensin system. Vitamin D could attenuate renal tubular cell damage following PM exposure by suppressing the renin-angiotensin system and by partially inhibiting the inflammatory response. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | D-RECEPTOR | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | AIR-POLLUTION | - |
dc.subject | D METABOLISM | - |
dc.subject | KIDNEY | - |
dc.subject | EXPRESSION | - |
dc.subject | PARTICLES | - |
dc.subject | PATHWAY | - |
dc.subject | ACTIVATION | - |
dc.title | Exposure to airborne particulate matter induces renal tubular cell injury in vitro: The role of vitamin D signaling and renin-angiotensin system | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Ju-Han | - |
dc.identifier.doi | 10.1016/j.heliyon.2022.e10184 | - |
dc.identifier.scopusid | 2-s2.0-85135966934 | - |
dc.identifier.wosid | 000865378400009 | - |
dc.identifier.bibliographicCitation | HELIYON, v.8, no.8 | - |
dc.relation.isPartOf | HELIYON | - |
dc.citation.title | HELIYON | - |
dc.citation.volume | 8 | - |
dc.citation.number | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | D-RECEPTOR | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | AIR-POLLUTION | - |
dc.subject.keywordPlus | D METABOLISM | - |
dc.subject.keywordPlus | KIDNEY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PARTICLES | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordAuthor | Airborne Particulate Matter | - |
dc.subject.keywordAuthor | Kidney Tubules | - |
dc.subject.keywordAuthor | Renin-Angiotensin System | - |
dc.subject.keywordAuthor | Vitamin D Deficiency | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.