Massively Parallel Selection of NanoCluster Beacons
DC Field | Value | Language |
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dc.contributor.author | Kuo, Yu-An | - |
dc.contributor.author | Jung, Cheulhee | - |
dc.contributor.author | Chen, Yu-An | - |
dc.contributor.author | Kuo, Hung-Che | - |
dc.contributor.author | Zhao, Oliver S. | - |
dc.contributor.author | Nguyen, Trung D. | - |
dc.contributor.author | Rybarski, James R. | - |
dc.contributor.author | Hong, Soonwoo | - |
dc.contributor.author | Chen, Yuan-, I | - |
dc.contributor.author | Wylie, Dennis C. | - |
dc.contributor.author | Hawkins, John A. | - |
dc.contributor.author | Walker, Jada N. | - |
dc.contributor.author | Shields, Samuel W. J. | - |
dc.contributor.author | Brodbelt, Jennifer S. | - |
dc.contributor.author | Petty, Jeffrey T. | - |
dc.contributor.author | Finkelstein, Ilya J. | - |
dc.contributor.author | Yeh, Hsin-Chih | - |
dc.date.accessioned | 2022-11-17T23:40:42Z | - |
dc.date.available | 2022-11-17T23:40:42Z | - |
dc.date.created | 2022-11-17 | - |
dc.date.issued | 2022-10 | - |
dc.identifier.issn | 0935-9648 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/145692 | - |
dc.description.abstract | NanoCluster Beacons (NCBs) are multicolor silver nanocluster probes whose fluorescence can be activated or tuned by a proximal DNA strand called the activator. While a single-nucleotide difference in a pair of activators can lead to drastically different activation outcomes, termed polar opposite twins (POTs), it is difficult to discover new POT-NCBs using the conventional low-throughput characterization approaches. Here, a high-throughput selection method is reported that takes advantage of repurposed next-generation-sequencing chips to screen the activation fluorescence of approximate to 40 000 activator sequences. It is found that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs and positions 4-6 and 2-4 are hotspots to generate yellow-orange and red POTs, respectively. Based on these findings, a "zipper-bag" model is proposed that can explain how these hotspots facilitate the formation of distinct silver cluster chromophores and alter their chemical yields. Combining high-throughput screening with machine-learning algorithms, a pipeline is established to design bright and multicolor NCBs in silico. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.subject | FLUORESCENT SILVER NANOCLUSTERS | - |
dc.subject | IONIZATION-MASS-SPECTROMETRY | - |
dc.subject | RNA-PROTEIN INTERACTIONS | - |
dc.subject | LABEL-FREE | - |
dc.subject | DNA | - |
dc.subject | OLIGONUCLEOTIDES | - |
dc.subject | RECOGNITION | - |
dc.subject | COMPLEXES | - |
dc.subject | CLUSTERS | - |
dc.subject | NUMBERS | - |
dc.title | Massively Parallel Selection of NanoCluster Beacons | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jung, Cheulhee | - |
dc.identifier.doi | 10.1002/adma.202204957 | - |
dc.identifier.scopusid | 2-s2.0-85137492105 | - |
dc.identifier.wosid | 000851859200001 | - |
dc.identifier.bibliographicCitation | ADVANCED MATERIALS, v.34, no.41 | - |
dc.relation.isPartOf | ADVANCED MATERIALS | - |
dc.citation.title | ADVANCED MATERIALS | - |
dc.citation.volume | 34 | - |
dc.citation.number | 41 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | FLUORESCENT SILVER NANOCLUSTERS | - |
dc.subject.keywordPlus | IONIZATION-MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | RNA-PROTEIN INTERACTIONS | - |
dc.subject.keywordPlus | LABEL-FREE | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | OLIGONUCLEOTIDES | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | CLUSTERS | - |
dc.subject.keywordPlus | NUMBERS | - |
dc.subject.keywordAuthor | fluorescent nanomaterials | - |
dc.subject.keywordAuthor | high-throughput screening | - |
dc.subject.keywordAuthor | NanoCluster Beacons | - |
dc.subject.keywordAuthor | next-generation sequencing | - |
dc.subject.keywordAuthor | silver nanoclusters | - |
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