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Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beadsopen access

Authors
Bae, MinjuPark, JunsooSeong, HyeonahLee, HansolChoi, WonsukNoh, JiyunKim, WoojooShin, Sehyun
Issue Date
8월-2022
Publisher
MDPI
Keywords
virus; nucleic acid; extraction; blade; lysis; magnetic beads
Citation
DIAGNOSTICS, v.12, no.8
Indexed
SCIE
SCOPUS
Journal Title
DIAGNOSTICS
Volume
12
Number
8
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/145851
DOI
10.3390/diagnostics12081995
ISSN
2075-4418
Abstract
The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and magnetic beads. The viral membrane can be instantly lysed using a high-speed rotating blade, and nucleic acids can be immediately isolated using a silica magnetic surface. The process was completed within 60 s by this method. Routine washing and eluting processes were subsequently conducted within 5 min. The results achieved by this method were comparable to those of a commercially available method. When the blade-based lysis and magnetic bead adsorption processes were performed separately, the RNA recovery rate was very low, and the Ct value was delayed compared to simultaneous lysis and RNA adsorption. Overall, this method not only dramatically shortens the conventional extraction time but also allows for its convenient use outside the laboratory, such as at remote field sites and for point-of-care testing.
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College of Medicine > Department of Medical Science > 1. Journal Articles
College of Engineering > Department of Mechanical Engineering > 1. Journal Articles

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