Pulmonary fibrosis model using micro-CT analyzable human PSC-derived alveolar organoids containing alveolar macrophage-like cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Seri | - |
dc.contributor.author | Choi, Jiwoong | - |
dc.contributor.author | Cheon, Seongmin | - |
dc.contributor.author | Song, Jihong | - |
dc.contributor.author | Kim, Seung-Yeon | - |
dc.contributor.author | Kim, Ji-eun | - |
dc.contributor.author | Nam, Dae-Hwan | - |
dc.contributor.author | Manzar, Gohar | - |
dc.contributor.author | Kim, Su-Man | - |
dc.contributor.author | Kang, Hyung-Sik | - |
dc.contributor.author | Kim, Kee K. | - |
dc.contributor.author | Jeong, Sang Hoon | - |
dc.contributor.author | Lee, Ju-Han | - |
dc.contributor.author | Park, Eun-Kee | - |
dc.contributor.author | Lee, Minseob | - |
dc.contributor.author | Lee, Hyang-Ae | - |
dc.contributor.author | Kim, Ki-Suk | - |
dc.contributor.author | Park, Han-Jin | - |
dc.contributor.author | Oh, Won Keun | - |
dc.contributor.author | Park, Chungoo | - |
dc.contributor.author | Lee, Chang Hyun | - |
dc.contributor.author | Kim, Eun-Mi | - |
dc.date.accessioned | 2022-11-19T06:40:19Z | - |
dc.date.available | 2022-11-19T06:40:19Z | - |
dc.date.created | 2022-11-17 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 0742-2091 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/145860 | - |
dc.description.abstract | Human lung organoids (hLOs) are useful for disease modelling and drug screening. However, a lack of immune cells in hLOs limits the recapitulation of in vivo cellular physiology. Here, we generated hLOs containing alveolar macrophage (AM phi)-like cells derived from pluripotent stem cells (PSC). To bridge hLOs with advanced human lung high-resolution X-ray computed tomography (CT), we acquired quantitative micro-CT images. Three hLO types were observed during differentiation. Among them, alveolar hLOs highly expressed not only lung epithelial cell markers but also AM phi-specific markers. Furthermore, CD68(+) AM phi-like cells were spatially organized on the luminal epithelial surface of alveolar hLOs. Bleomycin-treated alveolar hLOs showed upregulated expression of fibrosis-related markers and extracellular matrix deposits in the alveolar sacs. Alveolar hLOs also showed structural alterations such as excessive tissue fraction under bleomycin treatment. Therefore, we suggest that micro-CT analyzable PSC-derived alveolar hLOs are a promising in vitro model to predict lung toxicity manifestations, including fibrosis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.subject | HUMAN LUNG DEVELOPMENT | - |
dc.subject | STEM-CELLS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | MECHANISMS | - |
dc.subject | BLEOMYCIN | - |
dc.subject | PROVIDE | - |
dc.subject | DISEASE | - |
dc.title | Pulmonary fibrosis model using micro-CT analyzable human PSC-derived alveolar organoids containing alveolar macrophage-like cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Ju-Han | - |
dc.identifier.doi | 10.1007/s10565-022-09698-1 | - |
dc.identifier.scopusid | 2-s2.0-85126036883 | - |
dc.identifier.wosid | 000767017500001 | - |
dc.identifier.bibliographicCitation | CELL BIOLOGY AND TOXICOLOGY, v.38, no.4, pp.557 - 575 | - |
dc.relation.isPartOf | CELL BIOLOGY AND TOXICOLOGY | - |
dc.citation.title | CELL BIOLOGY AND TOXICOLOGY | - |
dc.citation.volume | 38 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 557 | - |
dc.citation.endPage | 575 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | HUMAN LUNG DEVELOPMENT | - |
dc.subject.keywordPlus | STEM-CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordPlus | BLEOMYCIN | - |
dc.subject.keywordPlus | PROVIDE | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordAuthor | Human alveolar organoid | - |
dc.subject.keywordAuthor | Fibrosis model | - |
dc.subject.keywordAuthor | Micro-CT | - |
dc.subject.keywordAuthor | Alveolar macrophage | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.