Visualization of differential GPCR crosstalk in DRD1-DRD2 heterodimer upon different dopamine levels
DC Field | Value | Language |
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dc.contributor.author | Kim, Hyunbin | - |
dc.contributor.author | Nam, Min-Ho | - |
dc.contributor.author | Jeong, Sohyeon | - |
dc.contributor.author | Lee, Hyowon | - |
dc.contributor.author | Oh, Soo-Jin | - |
dc.contributor.author | Kim, Jeongjin | - |
dc.contributor.author | Choi, Nakwon | - |
dc.contributor.author | Seong, Jihye | - |
dc.date.accessioned | 2022-11-19T12:40:20Z | - |
dc.date.available | 2022-11-19T12:40:20Z | - |
dc.date.created | 2022-11-17 | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 0301-0082 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/145920 | - |
dc.description.abstract | Dopaminergic signaling is regulated by transient micromolar (phasic) and background nanomolar (tonic) dopamine releases in the brain. These dopamine signals can be differentially translated by dopamine receptor type 1 and type 2, DRD1 and DRD2, which are G protein-coupled receptors (GPCRs). In response to dopamine, DRD1 and DRD2 are known to mediate opposite functions on cAMP production via Gs and Gi protein signaling. Interestingly, they can form a heterodimer. However, receptor crosstalk between DRD1-DRD2 heterodimers has not been directly measured, but it was only inferred from measuring downstream signaling pathways. Here we develop fluorescent protein-based multicolor biosensors which can monitor individual activation states of DRD1 and DRD2, and apply them to directly monitor the functional crosstalk between DRD1-DRD2 heterodimers in live cells. Utilizing these powerful tools, we surprisingly discover differential crosstalk in the DRD1-DRD2 heterodimers upon different dopamine (DA) levels: DRD1 activation is selectively inhibited at micromolar DA levels, while DRD2 is inhibited only by nanomolar DA concentration, implying a novel function of the DRD1-DRD2 heterodimer upon different DA levels. Our results imply differential receptor crosstalk and novel functions of the DRD1-DRD2 heterodimer in response to physiological dopamine levels from nanomolar to micromolar dopamine concentrations. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | D1-D2 RECEPTOR HETEROMER | - |
dc.subject | AFFINITY | - |
dc.subject | PHARMACOLOGY | - |
dc.subject | ACTIVATION | - |
dc.subject | NEURONS | - |
dc.subject | D-1 | - |
dc.subject | D1 | - |
dc.subject | HETEROOLIGOMERS | - |
dc.subject | RELEASE | - |
dc.subject | SYSTEM | - |
dc.title | Visualization of differential GPCR crosstalk in DRD1-DRD2 heterodimer upon different dopamine levels | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Nakwon | - |
dc.identifier.doi | 10.1016/j.pneurobio.2022.102266 | - |
dc.identifier.scopusid | 2-s2.0-85127784565 | - |
dc.identifier.wosid | 000805977700003 | - |
dc.identifier.bibliographicCitation | PROGRESS IN NEUROBIOLOGY, v.213 | - |
dc.relation.isPartOf | PROGRESS IN NEUROBIOLOGY | - |
dc.citation.title | PROGRESS IN NEUROBIOLOGY | - |
dc.citation.volume | 213 | - |
dc.type.rims | ART | - |
dc.type.docType | Review | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.subject.keywordPlus | D1-D2 RECEPTOR HETEROMER | - |
dc.subject.keywordPlus | AFFINITY | - |
dc.subject.keywordPlus | PHARMACOLOGY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | D-1 | - |
dc.subject.keywordPlus | D1 | - |
dc.subject.keywordPlus | HETEROOLIGOMERS | - |
dc.subject.keywordPlus | RELEASE | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | GPCR heterodimer | - |
dc.subject.keywordAuthor | Dopamine receptor sensor | - |
dc.subject.keywordAuthor | DRD1 | - |
dc.subject.keywordAuthor | DRD2 | - |
dc.subject.keywordAuthor | cAMP | - |
dc.subject.keywordAuthor | Tonic and phasic DA release | - |
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