Surface display of enzyme complex on Corynebacterium glutamicum as a whole cell biocatalyst and its consolidated bioprocessing using fungal-pretreated lignocellulosic biomass
DC Field | Value | Language |
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dc.contributor.author | Lee, M.-E. | - |
dc.contributor.author | Ko, Y.J. | - |
dc.contributor.author | Hwang, D.-H. | - |
dc.contributor.author | Cho, B.-H. | - |
dc.contributor.author | Jeong, W.-Y. | - |
dc.contributor.author | Bhardwaj, N. | - |
dc.contributor.author | Han, S.O. | - |
dc.date.accessioned | 2022-11-19T20:40:32Z | - |
dc.date.available | 2022-11-19T20:40:32Z | - |
dc.date.created | 2022-11-18 | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/146001 | - |
dc.description.abstract | A novel whole cell biocatalyst using fungal-pretreated lignocellulosic biomass was developed by displaying the enzyme complex consisting of N-acetylglucosaminidase (cNAG) and endoglucanse E (cCelE) on Corynebacterium glutamicum, hereafter called mNC. mNC showed a maximum 4.43-fold cNAG and 2.40-fold cCelE activity compared to single enzyme-secreting C. glutamicum. mNC also showed the highest efficiency of sugar production in various types of cellulose and fungal-pretreated biomass. The growth of mNC was 5.06-fold higher than that of the control. Then, the ability of mNC to produce a valuable chemical was confirmed. mNC overexpressing isopropanol biosynthesis genes showed a maximum titer of 218.9 ± 11.73 mg/L isopropanol and maintained high efficiency for isopropanol production in the recycling test, which was 90.07 ± 4.12 % during 4 cycles. This strategy can be applied to the direct saccharification of fungal-pretreated lignocellulosic biomass efficiently leading to the production of valuable products in various industrial fields. © 2022 Elsevier Ltd | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Surface display of enzyme complex on Corynebacterium glutamicum as a whole cell biocatalyst and its consolidated bioprocessing using fungal-pretreated lignocellulosic biomass | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Han, S.O. | - |
dc.identifier.doi | 10.1016/j.biortech.2022.127758 | - |
dc.identifier.scopusid | 2-s2.0-85135812136 | - |
dc.identifier.bibliographicCitation | Bioresource Technology, v.362 | - |
dc.relation.isPartOf | Bioresource Technology | - |
dc.citation.title | Bioresource Technology | - |
dc.citation.volume | 362 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Cellulase | - |
dc.subject.keywordAuthor | Cellulosome | - |
dc.subject.keywordAuthor | Corynebacterium glutamicum | - |
dc.subject.keywordAuthor | Isopropanol | - |
dc.subject.keywordAuthor | N-Acetylglucosaminidase | - |
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