Resveratrol-loaded gold nanoparticles enhance caspase-mediated apoptosis in PANC-1 pancreatic cells via mitochondrial intrinsic apoptotic pathway
DC Field | Value | Language |
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dc.contributor.author | Lee, Dong Gun | - |
dc.contributor.author | Lee, Mindong | - |
dc.contributor.author | Go, Eun Byeol | - |
dc.contributor.author | Chung, Namhyun | - |
dc.date.accessioned | 2022-12-08T14:41:46Z | - |
dc.date.available | 2022-12-08T14:41:46Z | - |
dc.date.created | 2022-12-08 | - |
dc.date.issued | 2022-12 | - |
dc.identifier.issn | 1868-6958 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/146487 | - |
dc.description.abstract | Background Pancreatic ductal adenocarcinoma (PDAC) remains one of the most fatal malignancies. Several chemotherapies employing fluorouracil (5-FU) and gemcitabine were attempted, but the survival rate was extremely low. Resveratrol (RVT), known as a polyphenol compound and phytoalexin, was demonstrated to induce intrinsic apoptosis in cancer cells. However, its low delivery performance and efficiency at tumor sites remain an obstacle to exploit RVT as a drug. To address these problems, we bio-conjugated resveratrol with gold nanoparticles (GNPs) via polyvinylpyrrolidone as a cross-linker (RVT@PVP-GNPs) and investigated whether the fabrications could enhance the delivery performance and anti-tumor efficacy of RVT. Results The fabrication of gold nanoparticles (GNPs) and bio-conjugated with resveratrol (RVT@PVP-GNPs) was conducted firstly. TEM image, spectrophotometry and zeta-potential revealed that the GNPs and RVT@PVP-GNPs having a size of approximately 40 nm were successfully synthesized and exhibited moderate stability. GNPs alone represented no damage in PANC-1 cells and moreover diminished the cytotoxicity of RVT in Raw264.7 murine macrophage cells, demonstrating the superiority of gold nanoparticles as a drug carrier. Evaluation using dialysis showed a burst release rate of RVT within 96 h at pH 5.0, demonstrating the possibility of enhanced efficiency of RVT delivery through blood vessels to the tumor. The RVT@PVP-GNPs induced increased rates of S-phase cell cycle arrest and apoptosis compared with free RVT. Notably, RVT@PVP-GNPs diminished the proportion of necrotic cells, whereas free RVT increased it. We also demonstrated that the RVT@PVP-GNPs may induce an apoptosis via intrinsic mitochondria with higher degree compared with free RVT, indicating the possibility of enhanced anti-tumor agents. In animal studies, RVT@PVP-GNPs conjugated with AS1411 aptamer induced efficient tumor volume suppression without accumulation in or damage to the kidneys in vivo. Conclusions The results demonstrate that RVT@PVP-GNPs enhance the anti-tumor efficacy of free RVT by activating the intrinsic apoptotic pathway and could be considered as potential anti-tumor drug candidates against pancreatic cancer cells. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | DEATH | - |
dc.subject | EXPRESSION | - |
dc.subject | CLEAVAGE | - |
dc.subject | NECROSIS | - |
dc.subject | HYPOXIA | - |
dc.subject | SIZE | - |
dc.subject | P53 | - |
dc.title | Resveratrol-loaded gold nanoparticles enhance caspase-mediated apoptosis in PANC-1 pancreatic cells via mitochondrial intrinsic apoptotic pathway | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chung, Namhyun | - |
dc.identifier.doi | 10.1186/s12645-022-00143-w | - |
dc.identifier.scopusid | 2-s2.0-85140630364 | - |
dc.identifier.wosid | 000874920900001 | - |
dc.identifier.bibliographicCitation | CANCER NANOTECHNOLOGY, v.13, no.1 | - |
dc.relation.isPartOf | CANCER NANOTECHNOLOGY | - |
dc.citation.title | CANCER NANOTECHNOLOGY | - |
dc.citation.volume | 13 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CLEAVAGE | - |
dc.subject.keywordPlus | NECROSIS | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | SIZE | - |
dc.subject.keywordPlus | P53 | - |
dc.subject.keywordAuthor | Anti-tumor effect | - |
dc.subject.keywordAuthor | Cell cycle | - |
dc.subject.keywordAuthor | Gold nanoparticles | - |
dc.subject.keywordAuthor | Intrinsic apoptosis | - |
dc.subject.keywordAuthor | Nano-medicine | - |
dc.subject.keywordAuthor | Pancreatic cancer | - |
dc.subject.keywordAuthor | Resveratrol | - |
dc.subject.keywordAuthor | S-phase arrest | - |
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