Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Seong, HyeonAh | - |
dc.contributor.author | Park, Junsoo | - |
dc.contributor.author | Bae, Minju | - |
dc.contributor.author | Shin, Sehyun | - |
dc.date.accessioned | 2022-12-09T16:00:36Z | - |
dc.date.available | 2022-12-09T16:00:36Z | - |
dc.date.created | 2022-12-08 | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | 2227-9059 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/146614 | - |
dc.description.abstract | Since its discovery in circulating blood seven decades ago, cell-free DNA (cfDNA) has become a highly focused subject in cancer management using liquid biopsy. Despite its clinical utility, the extraction of cfDNA from blood has many technical difficulties, including a low efficiency of recovery and long processing times. We introduced a magnetic bead-based cfDNA extraction method using homobifunctional crosslinkers, including dimethyl suberimidate dihydrochloride (DMS). Owing to its bifunctional nature, DMS can bind to DNA through either covalent or electrostatic bonding. By adopting amine-conjugated magnetic beads, DMS-DNA complexes can be rapidly isolated from blood plasma. Using standard washing and eluting processes, we successfully extracted cfDNA from plasma within 10 min. This method yielded a 56% higher extraction efficiency than that of a commercial product (QIAamp kit). Furthermore, the instant binding mechanism of amine coupling between the microbeads and DMS-DNA complexes significantly reduced the processing time. These results highlight the potential of this magnetic bead-based homobifunctional crosslinker platform for extraction of cfDNA from blood plasma. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | CANCER | - |
dc.subject | SERUM | - |
dc.subject | KITS | - |
dc.title | Rapid and Efficient Extraction of Cell-Free DNA Using Homobifunctional Crosslinkers | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Sehyun | - |
dc.identifier.doi | 10.3390/biomedicines10081883 | - |
dc.identifier.scopusid | 2-s2.0-85137316391 | - |
dc.identifier.wosid | 000846046600001 | - |
dc.identifier.bibliographicCitation | BIOMEDICINES, v.10, no.8 | - |
dc.relation.isPartOf | BIOMEDICINES | - |
dc.citation.title | BIOMEDICINES | - |
dc.citation.volume | 10 | - |
dc.citation.number | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | SERUM | - |
dc.subject.keywordPlus | KITS | - |
dc.subject.keywordAuthor | cfDNA | - |
dc.subject.keywordAuthor | extraction | - |
dc.subject.keywordAuthor | homobifunctional | - |
dc.subject.keywordAuthor | crosslinker | - |
dc.subject.keywordAuthor | magnetic beads | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.