Aquabacterium terrae sp. nov., isolated from soil

Abstract

A yellow-colored bacterial strain, designated S2(T) was isolated from soil in South Korea. Cells of strain S2(T) were strictly aerobic, Gram-stain-negative, motile with single polar flagellum, rod-shaped, oxidase and catalase-negative. Growth occurs at 10-37 degrees C (optimum, 28 degrees C), pH 5.0-9.0 (optimum, pH 6.5-7.0) and 0-3% NaCl (w/v). Strain S2(T) consisted of summed feature 3 (iso-C-15:0 2-OH and/or C-16:1 omega 7c), C-16:0 and summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) as major fatty acids. The sole respiratory quinone was Q-8. The polar lipid profile consisted of phosphatidylethanolamine and an unidentified lipid. The 16S rRNA gene sequence analysis showed that strain S2(T) is phylogenetically closest to Aquabacterium pictum W35(T) (98.4% sequence similarity). The genome of strain S2(T) was 8,039,486 bp with 56 scaffolds. The genome consisted of 10 putative biosynthetic gene clusters that are responsible for various secondary metabolites. Genomic DNA G + C content of strain S2(T) was 69.4%. The average nucleotide identity and in silico DNA-DNA hybridization values between strain S2(T) and phylogenetically related taxa were <= 77.9 and <= 21.4%, and respectively. The results of genotypic and phenotypic data showed that strain S2(T) could be distinguished from its phylogenetically related species and represents a novel species in the genus Aquabacterium, for which the name Aquabacterium terrae sp. nov. is proposed. The type strain is S2(T) (= KCTC 72741( T) = NBRC 114609( T)).