Fluorescent detection of dipicolinic acid as a biomarker in bacterial spores employing terbium ion-coordinated magnetite nanoparticles
- Authors
- Koo, Thomas Myeongseok; Ko, Min Jun; Park, Bum Chul; Kim, Myeong Soo; Kim, Young Keun
- Issue Date
- 15-4월-2021
- Publisher
- ELSEVIER
- Keywords
- Bacterial spore; Dipicolinic acid; Terbium ion; Magnetic nanoparticle; Fluorescence
- Citation
- JOURNAL OF HAZARDOUS MATERIALS, v.408
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF HAZARDOUS MATERIALS
- Volume
- 408
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/49422
- DOI
- 10.1016/j.jhazmat.2020.124870
- ISSN
- 0304-3894
- Abstract
- Anthrax is a bioterror agent because of its toxicity and the tolerance of its bacterial spores. Thus, researchers have attempted to develop various nanomaterials to detect dipicolinic acid (DPA), a biomarker of bacterial spores. Nanomaterials containing lanthanide ions have received considerable attention, owing to their potential to exhibit high sensitivity and selectivity in the detection of DPA via chelation with molecules. However, the fluorescent signals of the lanthanide complex are quenchable because the nanomaterials simultaneously absorb the excitation and emission light. For the precise detection of DPA, pure signals have to be obtained from the complex by alleviating the quenching effect of the nanomaterials. In this study, we develop a structure with terbium ion (Tb3+)-coordinated magnetite (Fe3O4) nanoparticle to detect DPA. Tb3+ can be detached from the magnetite during chelation with the DPA, and the complex can emit the unencumbered signals with improved detection limit through the application of a magnetic field. The detection system exhibits a significantly lower detection limit (5.4 nM) than the infectious dosage of anthrax (60 mu M) with high selectivity and chemical stability. This study informs the improvement of detection limits via the separation of nanomaterials and lanthanide complex.Y
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Engineering > Department of Materials Science and Engineering > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.