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Enhanced production of polyhydroxybutyrate from syngas by using nanoscaled cellulose particles with a syngas-converting enzyme complex immobilized on Ralstonia eutropha

Authors
Shin, Sang KyuKo, Young JinJeong, Da WoonLee, Myeong-EunYou, Seung KyouHyeon, Jeong EunHan, Sung Ok
Issue Date
20-2월-2021
Publisher
ELSEVIER SCI LTD
Keywords
Syngas utilization; Polyhydroxybutyrate; Enzyme complex; Nanoscaled cellulose particles; Ralstonia eutropha
Citation
JOURNAL OF CLEANER PRODUCTION, v.285
Indexed
SCIE
SCOPUS
Journal Title
JOURNAL OF CLEANER PRODUCTION
Volume
285
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/49556
DOI
10.1016/j.jclepro.2020.124903
ISSN
0959-6526
Abstract
Synthetic gas (syngas) produced from pyrolysis or a blast furnace is a mixture of different ratio of H-2, CO, N-2, CH4, CO2 and other carbon-containing gas. CO2 and H 2 are the key sources for Ralstonia eutropha, which uses CO2 to produce polyhydroxybutyrate (PHB) with a H-2 uptake system. For the efficient conversion of syngas to accessible carbon source, nanoscaled cellulose particles with an enzyme complex consisting of carbon monoxide dehydrogenase (CooS), carbon monoxide binding unit (CooA) and carbonic anhydrase (CA) were assembled by minimized scaffoldin (mCbpA). In addition to this assembly, mCbpA-BspA (MB) established to bridge the nanoparticle assembly and R. eutropha was used to provide higher enzyme stability and activity. The highest PHB accumulation after 72 h of fermentation was 14.2 g/L in a closed serum bottle that contained the enzyme assembly immobilized on R. eutropha. This catalytic assembly may be a new potential conversion tool to produce biodegradable PHB from syngas. (C) 2020 Elsevier Ltd. All rights reserved.
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