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Label-free multimodal microscopy using a single light source and detector for biological imaging

Authors
Kang, JuehyungKang, UngyoNam, Hyeong SooKim, WooseopKim, Hyun JungKim, Ryeong HyeonKim, Jin WonYoo, Hongki
Issue Date
15-2월-2021
Publisher
OPTICAL SOC AMER
Citation
OPTICS LETTERS, v.46, no.4, pp.892 - 895
Indexed
SCIE
SCOPUS
Journal Title
OPTICS LETTERS
Volume
46
Number
4
Start Page
892
End Page
895
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/49581
DOI
10.1364/OL.415938
ISSN
0146-9592
Abstract
Multimodal nonlinear microscopy has been widely applied in biology and medicine due to its relatively deep penetration into tissue and its label-free manner. However, current multimodal systems require the use of multiple sources and detectors, leading to bulky, complex, and expensive systems. In this Letter, we present a novel method of using a single light source and detector for nonlinear multimodal imaging of biological samples. Using a photonic crystal fiber, a pulse picker, and multimode fibers, our developed system successfully acquired multimodal images of swine coronary arteries, including two-photon excitation fluorescence, second-harmonic generation, coherent anti-Stokes Raman scattering, and backreflection. The developed system could be a valuable tool for various biomedical applications. (C) 2021 Optical Society of America
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