Ozonation enhancement of low cost double-stranded DNA binding dye based fluorescence measurement of total bacterial load in water
DC Field | Value | Language |
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dc.contributor.author | Choi, Jiwon | - |
dc.contributor.author | Chua, Beelee | - |
dc.contributor.author | Son, Ahjeong | - |
dc.date.accessioned | 2021-08-30T03:58:16Z | - |
dc.date.available | 2021-08-30T03:58:16Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2021-01-29 | - |
dc.identifier.issn | 2046-2069 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/50052 | - |
dc.description.abstract | We demonstrated the feasibility of using ozonation to enhance the performance of dsDNA binding dye SYBR Green I in the fluorescence measurement of total bacterial load in water. Unlike its membrane permeable but expensive equivalent such as SYTO82 dye, SYBR Green I is many times cheaper but membrane impermeable. Ozonation allowed SYBR Green I dye to permeate the membrane and bind with the dsDNA within by first breaching it. Using E. coli K12 bacteria at serial dilution ratios from 1/1 (980 CFU mL(-1)) to 1/200, we achieved corresponding quantification from 618.7 +/- 9.4 to 68.0 +/- 1.9 RFU (100 to 11.00% normalized RFU). In comparison, plate counting and optical density measurement were only able to quantify up till a serial dilution ratio of 1/50 (40 CFU mL(-1) and 0.0421, respectively). Most importantly with ozonation, the sensitivity of SYBR Green I dye based fluorescence measurement was improved by similar to 140 to 210% as compared to that without ozonation. Given its low electrical power consumption, lab-on-chip compatibility and reagent-less nature, ozonation is highly compatible with portable fluorimeters to realize low-cost monitoring of total bacterial load in water. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Ozonation enhancement of low cost double-stranded DNA binding dye based fluorescence measurement of total bacterial load in water | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Chua, Beelee | - |
dc.identifier.doi | 10.1039/d0ra08742d | - |
dc.identifier.scopusid | 2-s2.0-85099932925 | - |
dc.identifier.wosid | 000615282100017 | - |
dc.identifier.bibliographicCitation | RSC ADVANCES, v.11, no.7, pp.3931 - 3941 | - |
dc.relation.isPartOf | RSC ADVANCES | - |
dc.citation.title | RSC ADVANCES | - |
dc.citation.volume | 11 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 3931 | - |
dc.citation.endPage | 3941 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
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