PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE 1 modulates flowering in a florigen-independent manner by regulating SVP
- Authors
- Susila, Hendry; Nasim, Zeeshan; Gawarecka, Katarzyna; Jung, Ji-Yul; Jin, Suhyun; Youn, Geummin; Ahn, Ji Hoon
- Issue Date
- 1월-2021
- Publisher
- COMPANY BIOLOGISTS LTD
- Keywords
- Flowering; PECT1; SVP; GA20ox2; Gibberellin; Florigen; Arabidopsis
- Citation
- DEVELOPMENT, v.148, no.1
- Indexed
- SCIE
SCOPUS
- Journal Title
- DEVELOPMENT
- Volume
- 148
- Number
- 1
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/50628
- DOI
- 10.1242/dev.193870
- ISSN
- 0950-1991
- Abstract
- PHOSPHORYLETHANOLAMINE CYTIDYLYLTRANSFERASE 1 (PECT1) regulates phosphatidylethanolamine biosynthesis and controls the phosphatidylethanolamine:phosphatidylcholine ratio in Arabidopsis thaliana. Previous studies have suggested that PECT1 regulates flowering time by modulating the interaction between phosphatidylcholine and FLOWERING LOCUS T (Fr), a florigen, in the shoot apical meristem (SAM). Here, we show that knockdown of PECT1 by artificial microRNA in the SAM (pFD::amiR-PECT1) accelerated flowering under inductive and even non-inductive conditions, in which FT transcription is almost absent, and in ft-10 twin sister of ft-1 double mutants under both conditions. Transcriptome analyses suggested that PECT1 affects flowering by regulating SHORT VEGETATIVE PHASE (SVP) and GIBBERELLIN 20 OXIDASE 2 (GA20ox2). SVP misexpression in the SAM suppressed the early flowering of pFD::amiR-PECT1 plants. pFD::amiR-PECT1 plants showed increased gibberellin (GA) levels in the SAM, concomitant with the reduction of REPRESSOR OF GA1-3 levels. Consistent with this, GA treatment had little effect on flowering time of pFD::amiR-PECT1 plants and the GA antagonist paclobutrazol strongly affected flowering in these plants. Together, these results suggest that PECT1 also regulates flowering time through a florigen-independent pathway, modulating SVP expression and thus regulating GA production.
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Collections - College of Life Sciences and Biotechnology > Division of Life Sciences > 1. Journal Articles
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