Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7
DC Field | Value | Language |
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dc.contributor.author | Kim, Yeong Jun | - |
dc.contributor.author | Kim, Yuri | - |
dc.contributor.author | Kumar, Abhishek | - |
dc.contributor.author | Kim, Chan Woo | - |
dc.contributor.author | Toth, Zsolt | - |
dc.contributor.author | Cho, Nam Hyuk | - |
dc.contributor.author | Lee, Hye-Ra | - |
dc.date.accessioned | 2021-08-30T05:11:16Z | - |
dc.date.available | 2021-08-30T05:11:16Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2021-01 | - |
dc.identifier.issn | 1553-7366 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/50642 | - |
dc.description.abstract | Primary effusion lymphoma (PEL) is an aggressive B cell lymphoma that is etiologically linked to Kaposi's sarcoma-associated herpesvirus (KSHV). Despite standard multi-chemotherapy treatment, PEL continues to cause high mortality. Thus, new strategies to control PEL are needed urgently. Here, we show that a phosphodegron motif within the KSHV protein, latency-associated nuclear antigen (LANA), specifically interacts with E3 ubiquitin ligase FBW7, thereby competitively inhibiting the binding of the anti-apoptotic protein MCL-1 to FBW7. Consequently, LANA-FBW7 interaction enhances the stability of MCL-1 by preventing its proteasome-mediated degradation, which inhibits caspase-3-mediated apoptosis in PEL cells. Importantly, MCL-1 inhibitors markedly suppress colony formation on soft agar and tumor growth of KSHV+PEL/BCBL-1 in a xenograft mouse model. These results strongly support the conclusion that high levels of MCL-1 expression enable the oncogenesis of PEL cells and thus, MCL-1 could be a potential drug target for KSHV-associated PEL. This work also unravels a mechanism by which an oncogenic virus perturbs a key component of the ubiquitination pathway to induce tumorigenesis. Author summary Primary effusion lymphoma (PEL), a highly aggressive B cell lymphoma, is associated with Kaposi's sarcoma-associated herpesvirus (KSHV). However, the underlying mechanisms that govern the aggressiveness of KSHV-associated PEL are poorly understood. Here, we demonstrate that KSHV LANA interacts with cellular ubiquitin E3 ligase FBW7, sequestering MCL-1 from FBW7, which reduces MCL-1 ubiquitination. As such, LANA potently stabilizes and increases MCL-1 protein, leading to inhibition of caspase-3-mediated apoptosis in PEL cells. Furthermore, MCL-1 inhibitors efficiently blocked PEL progression in mouse xenograft model. These results suggest that LANA acts as a proto-oncogene via deregulating tumor suppressor FBW7, which upregulates anti-apoptotic MCL-1 expression. This study suggests drugs that target MCL-1 may serve as an effective therapy against KSHV+ PEL. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | PRIMARY EFFUSION LYMPHOMA | - |
dc.subject | UBIQUITIN LIGASE | - |
dc.subject | TUMOR-SUPPRESSOR | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | CANCER | - |
dc.subject | INHIBITION | - |
dc.subject | APOPTOSIS | - |
dc.subject | PHOSPHORYLATION | - |
dc.subject | IDENTIFICATION | - |
dc.subject | MANIPULATION | - |
dc.title | Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Hye-Ra | - |
dc.identifier.doi | 10.1371/journal.ppat.1009179 | - |
dc.identifier.scopusid | 2-s2.0-85099836658 | - |
dc.identifier.wosid | 000612396100002 | - |
dc.identifier.bibliographicCitation | PLOS PATHOGENS, v.17, no.1 | - |
dc.relation.isPartOf | PLOS PATHOGENS | - |
dc.citation.title | PLOS PATHOGENS | - |
dc.citation.volume | 17 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalResearchArea | Parasitology | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Parasitology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.subject.keywordPlus | PRIMARY EFFUSION LYMPHOMA | - |
dc.subject.keywordPlus | UBIQUITIN LIGASE | - |
dc.subject.keywordPlus | TUMOR-SUPPRESSOR | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | MANIPULATION | - |
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