HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-kappa B and TLR4-Independent Akt Signaling
DC Field | Value | Language |
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dc.contributor.author | Jeon, Jisu | - |
dc.contributor.author | Lee, Yeji | - |
dc.contributor.author | Yu, Hyeonseung | - |
dc.contributor.author | Ha, Un-Hwan | - |
dc.date.accessioned | 2021-08-30T07:05:19Z | - |
dc.date.available | 2021-08-30T07:05:19Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/51330 | - |
dc.description.abstract | IL-27, a heterodimeric cytokine composed of the p28 subunit and Epstein-Barr virus-induced gene 3 (EBI3), acts as a potent immunosuppressant and thus limits pathogenic inflammatory responses. IL-27 is upregulated upon Pseudomonas aeruginosa infection in septic mice, increasing susceptibility to the infection and decreasing clearance of the pathogen. However, it remains unclear which P. aeruginosa-derived molecules promote production of IL-27. In this study, we explored the mechanism by which P. aeruginosa DnaK, a heat shock protein 70-like protein, induces EBI3 expression, thereby promoting production of IL-27. Upregulation of EBI3 expression did not lead to an increase in IL-35, which consists of the p35 subunit and EBI3. The IL-27 production in response to DnaK was biologically active, as reflected by stimulation of IL-10 production. DnaK-mediated expression of EBI3 was driven by two distinct signaling pathways, NF-kappa B and Akt. However, NF-kappa B is linked to TLR4-associated signaling pathways, whereas Akt is not. Taken together, our results reveal that P. aeruginosa DnaK potently upregulates EBI3 expression, which in turn drives production of the prominent anti-inflammatory cytokine IL-27, as a consequence of TLR4-dependent activation of NF-kappa B and TLR4-independent activation of the Akt signaling pathway. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.title | HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-kappa B and TLR4-Independent Akt Signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ha, Un-Hwan | - |
dc.identifier.doi | 10.3390/ijms21239194 | - |
dc.identifier.scopusid | 2-s2.0-85097110851 | - |
dc.identifier.wosid | 000597458800001 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.23 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.title | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | - |
dc.citation.volume | 21 | - |
dc.citation.number | 23 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordAuthor | Akt | - |
dc.subject.keywordAuthor | DnaK | - |
dc.subject.keywordAuthor | EBI3 | - |
dc.subject.keywordAuthor | IL-27 | - |
dc.subject.keywordAuthor | NF-& | - |
dc.subject.keywordAuthor | #954 | - |
dc.subject.keywordAuthor | B | - |
dc.subject.keywordAuthor | Pseudomonas aeruginosa | - |
dc.subject.keywordAuthor | TLR4 | - |
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