Prominin-1-Radixin axis controls hepatic gluconeogenesis by regulating PKA activity
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun | - |
dc.contributor.author | Yu, Dong-Min | - |
dc.contributor.author | Park, Jun Sub | - |
dc.contributor.author | Lee, Hwayeon | - |
dc.contributor.author | Kim, Jun-Seok | - |
dc.contributor.author | Kim, Hong Lim | - |
dc.contributor.author | Koo, Seung-Hoi | - |
dc.contributor.author | Lee, Jae-Seon | - |
dc.contributor.author | Lee, Sungsoo | - |
dc.contributor.author | Ko, Young-Gyu | - |
dc.date.accessioned | 2021-08-30T09:26:41Z | - |
dc.date.available | 2021-08-30T09:26:41Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-11-05 | - |
dc.identifier.issn | 1469-221X | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/51830 | - |
dc.description.abstract | Prominin-1 (Prom1) is a major cell surface marker of cancer stem cells, but its physiological functions in the liver have not been elucidated. We analyzed the levels of mRNA transcripts in serum-starved primary WT (Prom1(+/+)) and KO (Prom1(-/-)) mouse hepatocytes using RNA sequencing (RNA-seq) data, and found that CREB target genes were downregulated. This initial observation led us to determine that Prom1 deficiency inhibited cAMP response element-binding protein (CREB) activation and gluconeogenesis, but not cyclic AMP (cAMP) accumulation, in glucagon-, epinephrine-, or forskolin-treated liver tissues and primary hepatocytes, and mitigated glucagon-induced hyperglycemia. Because Prom1 interacted with radixin, Prom1 deficiency prevented radixin from localizing to the plasma membrane. Moreover, systemic adenoviral knockdown of radixin inhibited CREB activation and gluconeogenesis in glucagon-treated liver tissues and primary hepatocytes, and mitigated glucagon-elicited hyperglycemia. Based on these results, we conclude that Prom1 regulates hepatic PKA signaling via radixin functioning as an A kinase-anchored protein (AKAP). | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.subject | PROTEIN-KINASE-A | - |
dc.subject | POLYTOPIC MEMBRANE-PROTEIN | - |
dc.subject | STEM-CELL | - |
dc.subject | NEURAL PROGENITORS | - |
dc.subject | EPITHELIAL-CELLS | - |
dc.subject | CAMP SIGNALS | - |
dc.subject | RADIXIN | - |
dc.subject | PROMININ | - |
dc.subject | CD133 | - |
dc.subject | EXPRESSION | - |
dc.title | Prominin-1-Radixin axis controls hepatic gluconeogenesis by regulating PKA activity | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Koo, Seung-Hoi | - |
dc.contributor.affiliatedAuthor | Ko, Young-Gyu | - |
dc.identifier.doi | 10.15252/embr.201949416 | - |
dc.identifier.scopusid | 2-s2.0-85092160049 | - |
dc.identifier.wosid | 000575958800001 | - |
dc.identifier.bibliographicCitation | EMBO REPORTS, v.21, no.11 | - |
dc.relation.isPartOf | EMBO REPORTS | - |
dc.citation.title | EMBO REPORTS | - |
dc.citation.volume | 21 | - |
dc.citation.number | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | PROTEIN-KINASE-A | - |
dc.subject.keywordPlus | POLYTOPIC MEMBRANE-PROTEIN | - |
dc.subject.keywordPlus | STEM-CELL | - |
dc.subject.keywordPlus | NEURAL PROGENITORS | - |
dc.subject.keywordPlus | EPITHELIAL-CELLS | - |
dc.subject.keywordPlus | CAMP SIGNALS | - |
dc.subject.keywordPlus | RADIXIN | - |
dc.subject.keywordPlus | PROMININ | - |
dc.subject.keywordPlus | CD133 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | cAMP signaling | - |
dc.subject.keywordAuthor | gluconeogenesis | - |
dc.subject.keywordAuthor | Prominin& | - |
dc.subject.keywordAuthor | #8208 | - |
dc.subject.keywordAuthor | 1 | - |
dc.subject.keywordAuthor | protein kinase A | - |
dc.subject.keywordAuthor | radixin | - |
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