Characterization of CYP125A13, the First Steroid C-27 Monooxygenase from Streptomyces peucetius ATCC27952
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rimal, Hemraj | - |
dc.contributor.author | Subedi, Pradeep | - |
dc.contributor.author | Kim, Ki-Hwa | - |
dc.contributor.author | Park, Hyun | - |
dc.contributor.author | Lee, Jun Hyuck | - |
dc.contributor.author | Oh, Tae-Jin | - |
dc.date.accessioned | 2021-08-30T09:34:01Z | - |
dc.date.available | 2021-08-30T09:34:01Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/51898 | - |
dc.description.abstract | The characterization of cytochrome P450 CYP125A13 from Streptomyces peucetius was conducted using cholesterol as the sole substrate. The in vitro enzymatic assay utilizing putidaredoxin and putidaredoxin reductase from Pseudomonas putida revealed that CYP125A13 bound cholesterol and hydroxylated it. The calculated K-D value, catalytic conversion rates, and Km value were 56.92 +/- 11.28 mu M, 1.95 nmol min(-1) nmol(-1), and 11.3 +/- 2.8 mu M, respectively. Gas chromatography-mass spectrometry (GC-MS) analysis showed that carbon 27 of the cholesterol side-chain was hydroxylated, characterizing CYP125A13 as steroid C27-hydroxylase. The homology modeling and docking results also revealed the binding of cholesterol to the active site, facilitated by the hydrophobic amino acids and position of the C27-methyl group near heme. This orientation was favorable for the hydroxylation of the C27-methyl group, supporting the in vitro analysis. This was the first reported case of the hydroxylation of cholesterol at the C-27 position by Streptomyces P450. This study also established the catalytic function of CYP125A13 and provides a solid basis for further studies related to the catabolic potential of Streptomyces species. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | PHYTOSTEROL SIDE-CHAIN | - |
dc.subject | MICROBIAL-DEGRADATION | - |
dc.subject | FATTY-ACIDS | - |
dc.subject | CHOLESTEROL | - |
dc.subject | OXIDATION | - |
dc.subject | ENZYMES | - |
dc.subject | P450 | - |
dc.subject | MICROORGANISMS | - |
dc.subject | BIOSYNTHESIS | - |
dc.subject | MECHANISMS | - |
dc.title | Characterization of CYP125A13, the First Steroid C-27 Monooxygenase from Streptomyces peucetius ATCC27952 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Park, Hyun | - |
dc.identifier.doi | 10.4014/jmb.2007.07004 | - |
dc.identifier.scopusid | 2-s2.0-85097003554 | - |
dc.identifier.wosid | 000595945600012 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.30, no.11, pp.1750 - 1759 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 30 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1750 | - |
dc.citation.endPage | 1759 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002649656 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | PHYTOSTEROL SIDE-CHAIN | - |
dc.subject.keywordPlus | MICROBIAL-DEGRADATION | - |
dc.subject.keywordPlus | FATTY-ACIDS | - |
dc.subject.keywordPlus | CHOLESTEROL | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | P450 | - |
dc.subject.keywordPlus | MICROORGANISMS | - |
dc.subject.keywordPlus | BIOSYNTHESIS | - |
dc.subject.keywordPlus | MECHANISMS | - |
dc.subject.keywordAuthor | Streptomyces peucetius | - |
dc.subject.keywordAuthor | cytochrome P450 | - |
dc.subject.keywordAuthor | CYP125A13 | - |
dc.subject.keywordAuthor | 27-hydroxycholesterol | - |
dc.subject.keywordAuthor | regio-selective hydroxylation | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
145 Anam-ro, Seongbuk-gu, Seoul, 02841, Korea+82-2-3290-2963
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.