Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation
DC Field | Value | Language |
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dc.contributor.author | Kadam, Avinash A. | - |
dc.contributor.author | Shinde, Surendra K. | - |
dc.contributor.author | Ghodake, Gajanan S. | - |
dc.contributor.author | Saratale, Ganesh D. | - |
dc.contributor.author | Saratale, Rijuta G. | - |
dc.contributor.author | Sharma, Bharat | - |
dc.contributor.author | Hyun, Seunghun | - |
dc.contributor.author | Sung, Jung-Suk | - |
dc.date.accessioned | 2021-08-30T12:23:56Z | - |
dc.date.available | 2021-08-30T12:23:56Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 2073-4360 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/52549 | - |
dc.description.abstract | A surface-engineered nano-support for enzyme laccase-immobilization was designed by grafting the surface of halloysite nanotubes (HNTs) with Fe3O4 nanoparticles and chitosan. Herein, HNTs were magnetized (HNTs-M) by a cost-effective reduction-precipitation method. The synthesized HNTs-M were grafted with 0.25%, 0.5%, 1%, and 2% chitosan (HNTs-M-chitosan), respectively. Synthesized HNTs-M-chitosan (0.25%), HNTs-M-chitosan (0.5%), HNTs-M-chitosan (1%) and HNTs-M-chitosan (2%) were linked with glutaraldehyde (GTA) for laccase immobilization. Among these formulations, HNTs-M-chitosan (1%) exhibited the highest laccase immobilization with 95.13% activity recovery and 100.12 mg/g of laccase loading. The optimized material was characterized thoroughly by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HR-TEM), X-ray powder diffraction (XRD), thermal gravimetric analysis (TGA), and vibrating sample magnetometer (VSM) analysis. The immobilized laccase (HNTs-M-chitosan (1%)-GTA-Laccase) exhibited higher pH, temperature, and storage stabilities. The HNTs-M-chitosan (1%)-GTA-Laccase possesses excellent reusability capabilities. At the end of 10 cycles of the reusability experiment, HNTs-M-chitosan (1%)-GTA-Laccase retained 59.88% of its initial activity. The immobilized laccase was utilized for redox-mediated degradation of sulfamethoxazole (SMX), resulting in 41%, 59%, and 62% degradation of SMX in the presence of 2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), guaiacol (GUA), and syringaldehyde (SA), respectively. Repeated SMX degradation (57.10% after the sixth cycle) confirmed the potential of HNTs-M-chitosan (1%)-GTA-Laccase for environmental pollutant degradation. Thus, we successfully designed chitosan-based, rapidly separable super-magnetic nanotubes for efficacious enhancement of laccase biocatalysis, which can be applied as nano-supports for other enzymes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.subject | SUPPORT | - |
dc.title | Chitosan-Grafted Halloysite Nanotubes-Fe3O4 Composite for Laccase-Immobilization and Sulfamethoxazole-Degradation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hyun, Seunghun | - |
dc.identifier.doi | 10.3390/polym12102221 | - |
dc.identifier.scopusid | 2-s2.0-85092780179 | - |
dc.identifier.wosid | 000585217700001 | - |
dc.identifier.bibliographicCitation | POLYMERS, v.12, no.10 | - |
dc.relation.isPartOf | POLYMERS | - |
dc.citation.title | POLYMERS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | SUPPORT | - |
dc.subject.keywordAuthor | chitosan | - |
dc.subject.keywordAuthor | laccase-immobilization | - |
dc.subject.keywordAuthor | nano-engineered supports | - |
dc.subject.keywordAuthor | super-magnetic separation | - |
dc.subject.keywordAuthor | sulfamethoxazole degradation | - |
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