Global epigenomic analysis of KSHV-infected primary effusion lymphoma identifies functional MYC superenhancers and enhancer RNAs
DC Field | Value | Language |
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dc.contributor.author | Park, Angela | - |
dc.contributor.author | Oh, Soohwan | - |
dc.contributor.author | Jung, Kyle L. | - |
dc.contributor.author | Choi, Un Yung | - |
dc.contributor.author | Lee, Hye-Ra | - |
dc.contributor.author | Rosenfeld, Michael G. | - |
dc.contributor.author | Jung, Jae U. | - |
dc.date.accessioned | 2021-08-30T15:05:45Z | - |
dc.date.available | 2021-08-30T15:05:45Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-09-01 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/53209 | - |
dc.description.abstract | Enhancers play indispensable roles in cell proliferation and survival through spatiotemporally regulating gene transcription. Active enhancers and superenhancers often produce noncoding enhancer RNAs (eRNAs) that precisely control RNA polymerase II activity. Kaposi's sarcoma-associated herpesvirus (KSHV) is a human oncogenic gamma-2 herpesvirus that causes Kaposi's sarcoma and primary effusion lymphoma (PEL). It is well characterized that KSHV utilizes host epigenetic machineries to control the switch between two lifecycles, latency and lytic replication. However, how KSHV impacts host epigenome at different stages of viral lifecycle is not well understood. Using global run-on sequencing (GRO-seq) and chromatin-immunoprecipitation sequencing (ChIP-seq), we profiled the dynamics of host transcriptional regulatory elements dur- ing latency and lytic replication of KSHV-infected PEL cells. This revealed that a number of critical host genes for KSHV latency, including MYC proto-oncogene, were under the control of super- enhancers whose activities were globally repressed upon viral reactivation. The eRNA-expressing MYC superenhancers were located downstream of the MYC gene in KSHV-infected PELs and played a key role in MYC expression. RNAi-mediated depletion or dCas9- KRAB CRISPR inhibition of eRNA expression significantly reduced MYC mRNA level in PELs, as did the treatment of an epigenomic drug that globally blocks superenhancer function. Finally, while cellular IRF4 acted upon eRNA expression and superenhancer function for MYC expression during latency, KSHV viral IRF4 repressed cellular IRF4 expression, decreasing MYC expression and thereby, facilitating lytic replication. These results indicate that KSHV acts as an epigenomic driver that modifies host epigenomic status upon reactivation by effectively regulating host enhancer function. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATL ACAD SCIENCES | - |
dc.subject | SARCOMA-ASSOCIATED HERPESVIRUS | - |
dc.subject | CHROMATIN LANDSCAPE | - |
dc.subject | SUPER-ENHANCERS | - |
dc.subject | GENE-EXPRESSION | - |
dc.subject | REACTIVATION | - |
dc.subject | INHIBITION | - |
dc.subject | PROTEIN | - |
dc.subject | TRANSCRIPTION | - |
dc.subject | PATHWAYS | - |
dc.subject | IRF4 | - |
dc.title | Global epigenomic analysis of KSHV-infected primary effusion lymphoma identifies functional MYC superenhancers and enhancer RNAs | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Hye-Ra | - |
dc.identifier.doi | 10.1073/pnas.1922216117 | - |
dc.identifier.scopusid | 2-s2.0-85090508413 | - |
dc.identifier.wosid | 000572969700005 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.117, no.35, pp.21618 - 21627 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.title | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.citation.volume | 117 | - |
dc.citation.number | 35 | - |
dc.citation.startPage | 21618 | - |
dc.citation.endPage | 21627 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | SARCOMA-ASSOCIATED HERPESVIRUS | - |
dc.subject.keywordPlus | CHROMATIN LANDSCAPE | - |
dc.subject.keywordPlus | SUPER-ENHANCERS | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | REACTIVATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | TRANSCRIPTION | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | IRF4 | - |
dc.subject.keywordAuthor | Kaposi&apos | - |
dc.subject.keywordAuthor | s sarcoma-associated virus | - |
dc.subject.keywordAuthor | primary effusion lymphoma | - |
dc.subject.keywordAuthor | viral epigenetics | - |
dc.subject.keywordAuthor | viral genomics | - |
dc.subject.keywordAuthor | enhancer | - |
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