Mass spectrometry-based proteomics of single cells and organoids: The new generation of cancer research
- Authors
- Ntwali, P. V. Migisha; Heo, Chae Eun; Han, Jong Yoon; Chae, Soo Yeon; Kim, Minji; Vu, Hung M.; Kim, Min-Sik; Kim, Hugh I.
- Issue Date
- 9월-2020
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Mass spectrometry (MS); Proteomics; Cellular heterogeneity; 3D cell; Single cell
- Citation
- TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v.130
- Indexed
- SCIE
SCOPUS
- Journal Title
- TRAC-TRENDS IN ANALYTICAL CHEMISTRY
- Volume
- 130
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/53306
- DOI
- 10.1016/j.trac.2020.116005
- ISSN
- 0165-9936
- Abstract
- Mass spectrometry (MS)-based proteomics is becoming a widely used technique for proteome characterization. Remarkable improvements in the resolution and sensitivity of MS instruments have enabled MS to address an ever-increasing array of biological questions. Nevertheless, the intrinsic cellular heterogeneity of biological systems continues to be a hurdle in MS-based proteomics for cancer research. MS-based proteomics of 3D cellular models and single-cell systems are being used as an effective analysis technique to characterize cellular heterogeneity among cancer cells. Proteomic analysis of 3D cellular models and single-cell systems provides a correlation between cellular heterogeneity and patient-specific responses to chemotherapy drugs, leading to effective and less toxic cancer treatments. Here, we review the pioneering achievements in MS-based proteomic analysis of 3D cell models and single-cell systems. Additionally, we provide insights into the contribution of MS-based proteomics, from examining tumoroid and single-cell systems to the development of precision medicine for cancer treatment. (C) 2020 Elsevier B.V. All rights reserved.
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