Thermophilic L-fucose isomerase from Thermanaeromonas toyohensis for L-fucose synthesis from L-fuculose
DC Field | Value | Language |
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dc.contributor.author | Kim, In Jung | - |
dc.contributor.author | Kim, Kyoung Heon | - |
dc.date.accessioned | 2021-08-30T15:43:11Z | - |
dc.date.available | 2021-08-30T15:43:11Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-09 | - |
dc.identifier.issn | 1359-5113 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/53654 | - |
dc.description.abstract | The beneficial biological properties of L-fucose have extended its commercial application potential in pharmaceutical, cosmetic, and food industries. Enzymatic production of c-fucose with c-fucose isomerase (L-FucI) is considered a selective, green, and efficient strategy. Efficient sugar production requires thermophilic enzymes with increased reaction rate, reduced risk of microbial contamination, and high sugar solubility. No study has evaluated the applicability of thermophilic L-FucI for c-fucose production. In this study, we explored the biochemical properties of a thermostable L-FucI from Thermanaeromonas toyohensis (TtFucI) using L-fuculose as a substrate. The recombinant TtFucI exhibited thermophilicity and optimum activity at 70 degrees C. The specific activity, K-m, and k(cat) toward L-fuculose were 199.8 U/mg, 33.4 mM, and 901.7 s(-1), respectively. Mn2+ ions increased the activity of the enzyme by similar to 10 times and enhanced its thermal stability. Our study, on L-fucose synthesis by thermostable L-FucI, suggests the potential application of this enzyme for the industrial production of L-fucose. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | CONTAINING SULFATED POLYSACCHARIDES | - |
dc.subject | ISOMERIZES L-FUCOSE | - |
dc.subject | D-ARABINOSE | - |
dc.subject | SUBSTRATE-SPECIFICITY | - |
dc.subject | D-ALTROSE | - |
dc.subject | OLIGOSACCHARIDES | - |
dc.subject | PROLIFERATION | - |
dc.subject | STABILITY | - |
dc.subject | ACID | - |
dc.title | Thermophilic L-fucose isomerase from Thermanaeromonas toyohensis for L-fucose synthesis from L-fuculose | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Kyoung Heon | - |
dc.identifier.doi | 10.1016/j.procbio.2020.05.017 | - |
dc.identifier.scopusid | 2-s2.0-85086499933 | - |
dc.identifier.wosid | 000551342900014 | - |
dc.identifier.bibliographicCitation | PROCESS BIOCHEMISTRY, v.96, pp.131 - 137 | - |
dc.relation.isPartOf | PROCESS BIOCHEMISTRY | - |
dc.citation.title | PROCESS BIOCHEMISTRY | - |
dc.citation.volume | 96 | - |
dc.citation.startPage | 131 | - |
dc.citation.endPage | 137 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Chemical | - |
dc.subject.keywordPlus | CONTAINING SULFATED POLYSACCHARIDES | - |
dc.subject.keywordPlus | ISOMERIZES L-FUCOSE | - |
dc.subject.keywordPlus | D-ARABINOSE | - |
dc.subject.keywordPlus | SUBSTRATE-SPECIFICITY | - |
dc.subject.keywordPlus | D-ALTROSE | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | L-Fucose | - |
dc.subject.keywordAuthor | L- | - |
dc.subject.keywordAuthor | L-fucose isomerase | - |
dc.subject.keywordAuthor | Thermophilic | - |
dc.subject.keywordAuthor | Thermanaeromonas toyohensis | - |
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