Immobilization of laccase on a graphene interface: Direct electron transfer and molecular dynamics study
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Taeyoung | - |
dc.contributor.author | Baek, Inchul | - |
dc.contributor.author | Lee, Seonwoo | - |
dc.contributor.author | Choi, Hyunsung | - |
dc.contributor.author | Yoon, Seongho | - |
dc.contributor.author | Lee, Howon | - |
dc.contributor.author | Kim, Sun Ung | - |
dc.contributor.author | Na, Sungsoo | - |
dc.date.accessioned | 2021-08-30T16:51:30Z | - |
dc.date.available | 2021-08-30T16:51:30Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-08-15 | - |
dc.identifier.issn | 0169-4332 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/53764 | - |
dc.description.abstract | Direct electron transfer (DET) in biocatalysts and the interactions of biocatalysts at electrode interfaces are critical issues for the development of electrochemical devices. In comparison to high-performance complex electrodes, graphene-based electrodes have attracted significant attention based on their superior electrical conductivity, material properties, and low cost. However, the immobilization of laccase (LAC), an oxygen-reducing enzyme with high catalytic activity that is applied to cathodes, and interfaces formed between LAC and graphene have rarely been explored. In this study, electrochemical experiments employing cyclic voltammetry and electrochemical impedance spectroscopy were performed, and it was determined that graphene exhibits a maximum of a 1.57-fold increase in terms of its oxygen reduction rate compared to Au and carbon nanotubes. Additionally, DET rate revealed that graphene behaves more efficiently on immobilized LAC. Furthermore, absorbed morphologies were visualized, and computational methods were applied to verify binding sites, orientations, structures, and binding affinities in atomic scale. The axial ligands at T1 Cu sites were mutated using different hydrophobic amino acids, and the effects of mutation on interactions at interfaces were compared. Based on our experimental and theoretical results, LAC immobilization on graphene appears to be stronger than that on a charged surface without critical structural changes. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.subject | CHEMICALLY-MODIFIED ENZYMES | - |
dc.subject | WALLED CARBON NANOTUBES | - |
dc.subject | GLUCOSE-OXIDASE | - |
dc.subject | BIOFUEL CELLS | - |
dc.subject | TRAMETES-VERSICOLOR | - |
dc.subject | METAL-ELECTRODES | - |
dc.subject | COPPER | - |
dc.subject | OXIDATION | - |
dc.subject | ELECTROCHEMISTRY | - |
dc.subject | COMMUNICATION | - |
dc.title | Immobilization of laccase on a graphene interface: Direct electron transfer and molecular dynamics study | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Na, Sungsoo | - |
dc.identifier.doi | 10.1016/j.apsusc.2020.146378 | - |
dc.identifier.wosid | 000591924900001 | - |
dc.identifier.bibliographicCitation | APPLIED SURFACE SCIENCE, v.521 | - |
dc.relation.isPartOf | APPLIED SURFACE SCIENCE | - |
dc.citation.title | APPLIED SURFACE SCIENCE | - |
dc.citation.volume | 521 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Coatings & Films | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.relation.journalWebOfScienceCategory | Physics, Condensed Matter | - |
dc.subject.keywordPlus | CHEMICALLY-MODIFIED ENZYMES | - |
dc.subject.keywordPlus | WALLED CARBON NANOTUBES | - |
dc.subject.keywordPlus | GLUCOSE-OXIDASE | - |
dc.subject.keywordPlus | BIOFUEL CELLS | - |
dc.subject.keywordPlus | TRAMETES-VERSICOLOR | - |
dc.subject.keywordPlus | METAL-ELECTRODES | - |
dc.subject.keywordPlus | COPPER | - |
dc.subject.keywordPlus | OXIDATION | - |
dc.subject.keywordPlus | ELECTROCHEMISTRY | - |
dc.subject.keywordPlus | COMMUNICATION | - |
dc.subject.keywordAuthor | Direct electron transfer | - |
dc.subject.keywordAuthor | Laccase | - |
dc.subject.keywordAuthor | Graphene | - |
dc.subject.keywordAuthor | Electrochemistry | - |
dc.subject.keywordAuthor | Mutation effect | - |
dc.subject.keywordAuthor | Molecular dynamics | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.