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Novel peptides functionalized gold nanoparticles decorated tungsten disulfide nanoflowers as the electrochemical sensing platforms for the norovirus in an oyster

Authors
Baek, Seung HoonPark, Chan YeongThang Phan NguyenKim, Min WooPark, Jong PilChoi, ChangsunKim, Soo YoungKailasa, Suresh KumarPark, Tae Jung
Issue Date
8월-2020
Publisher
ELSEVIER SCI LTD
Keywords
Norovirus; Novel binding peptide; Nanobiosensor; Electrochemical impedance spectroscopy; Oyster
Citation
FOOD CONTROL, v.114
Indexed
SCIE
SCOPUS
Journal Title
FOOD CONTROL
Volume
114
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/53861
DOI
10.1016/j.foodcont.2020.107225
ISSN
0956-7135
Abstract
Human norovirus is one of the potential foodborne pathogens causing acute gastroenteritis, diarrhea, and food poisoning. For norovirus detection, we developed the norovirus specifically capturable peptides functionalized gold nanoparticles (AuNP) decorated tungsten disulfide nanoflower (WS2NF/AuNP). Based on the 3D-structured WS2NF acting as a supporting material, AuNP were immobilized on its surface to assist the conductivity of WS2NF and provide effective immobilization sites for bioreceptors. Since norovirus is composed of protein that functions as an insulant, as the virus bind with WS2NF/AuNP, the impedance is elevated by hindering the charge transfer between the working electrode and redox species ([Fe(CN)(6)](3-/)(4-)). The prepared nano-bio hybrid sensor was assessed by electrochemical impedance spectroscopic technique with spiked samples and deliberately infected oyster samples, respectively. In the case of the former, the electrochemical sensor represents a low detection limit (2.37 copies/mL) with a detection range from negative control to 10(4) copies/mL, and it was shown a detection limit of 6.21 copies/mL even in the oyster samples. Besides, when it applies to the different types of viruses such as rotavirus and dengue virus to verify the selectivity of this system, there were no effective signals except the norovirus. Therefore, it revealed that combining WS2NF and AuNP enhances electrochemical activity and stability via mutual synergetic effects.
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