Roles for Drosophila cap1 2 '-O-ribose methyltransferase in the small RNA silencing pathway associated with Argonaute 2
- Authors
- Lee, Seungjae; Hong, Jae-Sang; Lim, Do-Hwan; Lee, Young Sik
- Issue Date
- 8월-2020
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Drosophila; cap1; CMTR1; siRNA; Ago2; RISC
- Citation
- INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, v.123
- Indexed
- SCIE
SCOPUS
- Journal Title
- INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
- Volume
- 123
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54217
- DOI
- 10.1016/j.ibmb.2020.103415
- ISSN
- 0965-1748
- Abstract
- Cap1 2 '-O-ribose methyltransferase (CMTR1) modifies RNA transcripts containing the 7-methylguanosine cap via 2 '-O-ribose methylation of the first transcribed nucleotide, yielding cap1 structures. However, the role of CMTR1 in small RNA-mediated gene silencing remains unknown. Here, we identified and characterized a Drosophila CMTR1 gene (dCMTR1) mutation. We found that the catalytic activity of dCMTR1 was involved in the biogenesis of small interfering RNAs (siRNAs) but not microRNAs. Additionally, dCMTR1 interacted with R2D2, a key component for the assembly of the RNA-induced silencing complex (RISC) containing Argonaute 2 (Ago2). Consistent with this finding, loss of dCMTR1 function impaired RISC assembly by inhibiting the unwinding of Ago2-bound siRNA duplexes, thus preventing the retention of the guide strand. Moreover, dCMTR1 is unlikely to modify siRNAs during RISC assembly. Collectively, our data indicate that dCMTR1 is a positive regulator of the small RNA pathway associated with Ago2 with roles in both siRNA biogenesis and RISC assembly.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Life Sciences and Biotechnology > Division of Biotechnology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.