Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type
- Authors
- Lee, Jeoung Eun; Lee, Ji Yoon; Park, Chang-Hwan; Eum, Jin Hee; Jung, Soo Kyung; Han, A-Reum; Seol, Dong-Won; Lee, Jin Saem; Shin, Hyun Soo; Im, Jung Ho; Chun, Taehoon; Ha, Kyungsoo; Heo, Deok Rim; Yoon, Tae Ki; Lee, Dong Ryul
- Issue Date
- 14-7월-2020
- Publisher
- CELL PRESS
- Keywords
- frozen cord blood cells; frozen oocytes; homozygous HLA; pluripotent stem cells; somatic cell nuclear transfer
- Citation
- STEM CELL REPORTS, v.15, no.1, pp.171 - 184
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELL REPORTS
- Volume
- 15
- Number
- 1
- Start Page
- 171
- End Page
- 184
- URI
- https://scholar.korea.ac.kr/handle/2021.sw.korea/54400
- DOI
- 10.1016/j.stemcr.2020.05.005
- ISSN
- 2213-6711
- Abstract
- Human pluripotent stem cells (PSCs)through somatic cell nuclear transfer (SCNT) may be an important source for regenerative medicine. The low derivation efficiency of stem cells and the accessibility of human oocytes are the main obstacles to their application. We previously reported that the efficiency of SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a modified derivation method to the PSC line and first obtained human SCNT-PSC lines derived from both donated cryo-preserved oocytes and cord blood cells with a homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar characteristics with embryonic stem cells (ESCs) and additionally have shown immunocompatibility in an in vitro and in vivo humanized mouse with a matching HLA type. Our study demonstrates that SCNT technology using donated cryopreserved oocytes and cord blood cells with a known HLA type provides a promising method for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.
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Collections - Graduate School > Department of Biotechnology > 1. Journal Articles
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