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Aucklandia lappa Causes Cell Wall Damage in Candida albicans by Reducing Chitin and (1,3)-beta-D-Glucan

Authors
Lee, Heung-ShickKim, Younhee
Issue Date
7월-2020
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Keywords
Aucklandia lappa; Calcofluor white; Candida albicans; cell wall; chitin; (1,3)-beta-D-glucan
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.30, no.7, pp.967 - 973
Indexed
SCIE
SCOPUS
KCI
Journal Title
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume
30
Number
7
Start Page
967
End Page
973
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/54873
DOI
10.4014/jmb.2002.02025
ISSN
1017-7825
Abstract
The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-beta-D-glucan synthesis. A sorbitol protection assay demonstrated that the minimum inhibitory concentration (MIC) of the A. lappa extract against C. albicans cells increased eight-fold from 0.78 to 6.24 mg/ml in 72 h. Cell aggregates, which indicate damage to the cell wall or membrane, were commonly observed in the A. lappatreated C. albicans cells through microscopic analysis. In addition, the relative fluorescence intensities of the C. albicans cells incubated with the A. lappa extract for 3, 5, and 6 h were 92.1, 84.6, and 79.8%, respectively, compared to the controls, estimated by Calcofluor White binding assay. This result indicates that chitin content was reduced by the A. lappa treatment. Furthermore, synthesis of (1,3)-beta-D-glucan polymers was inhibited to 84.3, 79.7, and 70.2% of that of the control treatment following incubation of C. albicans microsomes with the A. lappa extract at a final concentration equal to its MIC, 2x MIC, and 4x MIC, respectively. These findings suggest that the A. lappa ethanol extract may aid the development of a new antifungal to successfully control Candida-associated disease.
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