Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus
DC Field | Value | Language |
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dc.contributor.author | Yoo, In Young | - |
dc.contributor.author | Kim, Ji-Youn | - |
dc.contributor.author | Yoon, Young Kyung | - |
dc.contributor.author | Huh, Hee Jae | - |
dc.contributor.author | Lee, Nam Yong | - |
dc.date.accessioned | 2021-08-30T20:26:40Z | - |
dc.date.available | 2021-08-30T20:26:40Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.issn | 2234-3814 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/54891 | - |
dc.description.abstract | The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed Huaiyangshan banyangvirus). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a-chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV. | - |
dc.format.extent | 4 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | KOREAN SOC LABORATORY MEDICINE | - |
dc.title | Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.3343/alm.2020.40.4.317 | - |
dc.identifier.scopusid | 2-s2.0-85079626974 | - |
dc.identifier.wosid | 000514836000005 | - |
dc.identifier.bibliographicCitation | ANNALS OF LABORATORY MEDICINE, v.40, no.4, pp 317 - 320 | - |
dc.citation.title | ANNALS OF LABORATORY MEDICINE | - |
dc.citation.volume | 40 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 317 | - |
dc.citation.endPage | 320 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002595622 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Medical Laboratory Technology | - |
dc.relation.journalWebOfScienceCategory | Medical Laboratory Technology | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | BUNYAVIRUS | - |
dc.subject.keywordPlus | DIAGNOSIS | - |
dc.subject.keywordPlus | ULTRAFAST | - |
dc.subject.keywordPlus | RNA | - |
dc.subject.keywordAuthor | Huaiyangshan banyangvirus | - |
dc.subject.keywordAuthor | SFTS virus | - |
dc.subject.keywordAuthor | Performance | - |
dc.subject.keywordAuthor | Turnaround time | - |
dc.subject.keywordAuthor | SFTS-QS kit | - |
dc.subject.keywordAuthor | PowerChek SFTSV Real-time PCR kit | - |
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