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Molecular and Histologic Evidence of Novel Erectile Dysfunction Rat Model as an Aging Atherosclerosis Model: A Preliminary Study

Authors
Kim, Jae HeonShim, Ji SungKim, Jong WookDoo, Seung WhanBae, Jae HyunLee, Ju HanSong, Yun SeobKim, Je JongMoon, Du Geon
Issue Date
7월-2020
Publisher
PUSAN NATL UNIV MEDICAL SCH, DEPT UROLOGY
Keywords
Atherosclerosis; Erectile dysfunction; Phosphodiesterase 5 Inhibitors; Vasculogenic impotence
Citation
WORLD JOURNAL OF MENS HEALTH, v.38, no.3, pp.345 - 352
Indexed
SCIE
SCOPUS
KCI
Journal Title
WORLD JOURNAL OF MENS HEALTH
Volume
38
Number
3
Start Page
345
End Page
352
URI
https://scholar.korea.ac.kr/handle/2021.sw.korea/54929
DOI
10.5534/wjmh.190031
ISSN
2287-4208
Abstract
Purpose: To validate a novel arteriogenic erectile dysfunction (ED) model with atherosclerosis (AS) based on molecular and histologic evidence induced by chronic pelvic ischemia (CPI) and determine effect of phosphodiesterase-5 inhibitor treatment. Materials and Methods: Twenty 16-week-old male Sprague-Dawley rats were divided into three experimental groups (Group I, untreated sham-operated rats with regular diet; Group II, CPI with cholesterol diet; Group III, CPI model with cholesterol diet and mirodenafil). Erectile function was accessed using maximum intracavernous pressure (ICP) and ICP/mean arterial pressure (MAP). Molecular changes were examined by western blot analysis using hypoxia inducible factor 1-alpha (HIF-1 alpha), endothelial nitric oxide synthase (eNOS), and transforming growth factor beta-1 (TGF-beta 1) antibodies. Collagen change was evaluated by Masson's trichrome staining. Results: In vivo measurements of ICP and ICP/MAP in Group II were significantly lower than those in Group I (p<0.01). Smooth muscle/collagen ratio in Group II was significantly lower than that in Group I (p<0.05). After treatment with mirodenafil for four weeks, Group III showed significantly higher levels of ICP and ICP/MAP than Group II (p<0.05). Western blot analysis showed that HIF-1 alpha and TGF-beta 1 levels were significantly higher in Group II whereas eNOS levels were significantly lower in Group II than those in Group I or III. Conclusions: A novel arteriogenic ED with AS model is successfully induced by CPI and validated based on molecular and histologic evidences.
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