Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bang, Jinho | - |
dc.contributor.author | Park, Heesun | - |
dc.contributor.author | Yoo, Jihye | - |
dc.contributor.author | Lee, Donghyun | - |
dc.contributor.author | Choi, Won Il | - |
dc.contributor.author | Lee, Jin Hyung | - |
dc.contributor.author | Lee, Young-Ran | - |
dc.contributor.author | Kim, Chungho | - |
dc.contributor.author | Koo, Heebeom | - |
dc.contributor.author | Kim, Sunghyun | - |
dc.date.accessioned | 2021-08-30T20:36:05Z | - |
dc.date.available | 2021-08-30T20:36:05Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-06-29 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/54961 | - |
dc.description.abstract | The global burden of bone-related diseases is increasing in the aging society; thus, improved bone targeted imaging for their early identification and treatment are needed. In this study, we screened novel peptide ligands for hydroxyapatite, a major inorganic component of teeth and bones, and identified a peptide enabling in vivo bone targeting and real-time fluorescence bone detection. To isolate peptides highly specific for hydroxyapatite, we used negative and positive selection from a randomized 8-mer peptide phage library and identified hydroxyapatite-specific peptides (HA-pep2, HA-pep3, and HA-pep7). Among these three peptides, HA-pep3 showed the highest binding capacity and superior dissociation constant towards hydroxyapatite surfaces over time (similar to 88.3% retained on hydroxyapatite after two weeks). Furthermore, HA-pep3 was highly specific for hydroxyapatite compared to other calcium salt-based materials. Using this superior specificity, HA-pep3 showed higher accumulation in skull, spine, and joints in comparison with scrambled control peptide during real-time whole-body imaging. Ex vivo analysis of the major organs and bone from mice demonstrated that the fluorescence intensity in bone was about 3.32 folds higher in the case of HA-pep3 than the one exhibited by the scrambled control peptide. Our study identified a novel approach for targeting ligands for bone specific imaging and can be useful for drug delivery applications. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | NATURE RESEARCH | - |
dc.subject | NEAR-INFRARED FLUOROPHORES | - |
dc.subject | MODULAR PEPTIDES | - |
dc.subject | HYDROXYAPATITE | - |
dc.subject | DELIVERY | - |
dc.subject | BINDING | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | BIOMATERIALS | - |
dc.subject | ENHANCEMENT | - |
dc.title | Selection and identification of a novel bone-targeting peptide for biomedical imaging of bone | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Chungho | - |
dc.identifier.doi | 10.1038/s41598-020-67522-4 | - |
dc.identifier.scopusid | 2-s2.0-85087013340 | - |
dc.identifier.wosid | 000548360700011 | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.10, no.1 | - |
dc.relation.isPartOf | SCIENTIFIC REPORTS | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | NEAR-INFRARED FLUOROPHORES | - |
dc.subject.keywordPlus | MODULAR PEPTIDES | - |
dc.subject.keywordPlus | HYDROXYAPATITE | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | BIOMATERIALS | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
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