Transcriptional activities of human elongation factor-1 alpha and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Eun, Kiyoung | - |
dc.contributor.author | Hong, Nayoung | - |
dc.contributor.author | Jeong, Yeon Woo | - |
dc.contributor.author | Park, Min Gi | - |
dc.contributor.author | Hwang, Seon-Ung | - |
dc.contributor.author | Jeong, Yeon I. K. | - |
dc.contributor.author | Choi, Eun Ji | - |
dc.contributor.author | Olsson, P. Olof | - |
dc.contributor.author | Hwang, Woo Suk | - |
dc.contributor.author | Hyun, Sang-Hwan | - |
dc.contributor.author | Kim, Hyunggee | - |
dc.date.accessioned | 2021-08-30T21:27:10Z | - |
dc.date.available | 2021-08-30T21:27:10Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-06-03 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/55055 | - |
dc.description.abstract | Recent advances in somatic cell nuclear transfer (SCNT) in canines facilitate the production of canine transgenic models. Owing to the importance of stable and strong promoter activity in transgenic animals, we tested human elongation factor 1 alpha (hEF1 alpha) and cytomegalovirus (CMV) promoter sequences in SCNT transgenic dogs. After transfection, transgenic donor fibroblasts with the hEF1 alpha-enhanced green fluorescence protein (EGFP) transgene were successfully isolated using fluorescence-activated cell sorting (FACS). We obtained four puppies, after SCNT, and identified three puppies as being transgenic using PCR analysis. Unexpectedly, EGFP regulated by hEF1 alpha promoter was not observed at the organismal and cellular levels in these transgenic dogs. EGFP expression was rescued by the inhibition of DNA methyltransferases, implying that the hEF1 alpha promoter is silenced by DNA methylation. Next, donor cells with CMV-EGFP transgene were successfully established and SCNT was performed. Three puppies of six born puppies were confirmed to be transgenic. Unlike hEF1 alpha-regulated EGFP, CMV-regulated EGFP was strongly detectable at both the organismal and cellular levels in all transgenic dogs, even after 19 months. In conclusion, our study suggests that the CMV promoter is more suitable, than the hEF1 alpha promoter, for stable transgene expression in SCNT-derived transgenic canine model. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | EPIGENETIC REGULATION | - |
dc.subject | EXPRESSION | - |
dc.subject | MODEL | - |
dc.subject | METHYLATION | - |
dc.subject | PREGNANCY | - |
dc.subject | VERSATILE | - |
dc.subject | PATTERNS | - |
dc.subject | VECTORS | - |
dc.title | Transcriptional activities of human elongation factor-1 alpha and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Hyunggee | - |
dc.identifier.doi | 10.1371/journal.pone.0233784 | - |
dc.identifier.scopusid | 2-s2.0-85085908576 | - |
dc.identifier.wosid | 000539293000011 | - |
dc.identifier.bibliographicCitation | PLOS ONE, v.15, no.6 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.citation.title | PLOS ONE | - |
dc.citation.volume | 15 | - |
dc.citation.number | 6 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | EPIGENETIC REGULATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | METHYLATION | - |
dc.subject.keywordPlus | PREGNANCY | - |
dc.subject.keywordPlus | VERSATILE | - |
dc.subject.keywordPlus | PATTERNS | - |
dc.subject.keywordPlus | VECTORS | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.