A JUN N-terminal kinase inhibitor induces ectodomain shedding of the cancer-associated membrane protease Prss14/epithin via protein kinase C?II
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoon, Joobyoung | - |
dc.contributor.author | Cho, Youngkyung | - |
dc.contributor.author | Kim, Ki Yeon | - |
dc.contributor.author | Yoon, Min Ji | - |
dc.contributor.author | Lee, Hyo Seon | - |
dc.contributor.author | Jeon, Sangjun Davie | - |
dc.contributor.author | Cho, Yongcheol | - |
dc.contributor.author | Kim, Chungho | - |
dc.contributor.author | Kim, Moon Gyo | - |
dc.date.accessioned | 2021-08-30T23:59:09Z | - |
dc.date.available | 2021-08-30T23:59:09Z | - |
dc.date.created | 2021-06-19 | - |
dc.date.issued | 2020-05-15 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/55682 | - |
dc.description.abstract | Serine protease 14 (Prss14)/epithin is a transmembrane serine protease that plays essential roles in tumor progression and metastasis and therefore is a promising target for managing cancer. Prss14/epithin shedding may underlie its activity in cancer and worsen outcomes; accordingly, a detailed understanding of the molecular mechanisms in Prss14/epithin shedding may inform the design of future cancer therapies. On the basis of our previous observation that an activator of PKC, phorbol 12-myristate 13-acetate (PMA), induces Prss14/epithin shedding, here we further investigated the intracellular signaling pathway involved in this process. While using mitogen-activated protein kinase inhibitors to investigate possible effectors of downstream PKC signaling, we unexpectedly found that an inhibitor of c-Jun N-terminal kinase (JNK), SP600125, induces Prss14/epithin shedding even in the absence of PMA. SP600125-induced shedding, like that stimulated by PMA, was mediated by tumor necrosis factor-??converting enzyme. In contrast, a JNK activator, anisomycin, partially abolished the effects of SP600125 on Prss14/epithin shedding. Moreover, the results from loss-of-function experiments with specific inhibitors, short hairpin RNA?mediated knockdown, and overexpression of dominant-negative PKC?II variants indicated that PKC?II is a major player in JNK inhibition? and PMA-mediated Prss14/epithin shedding. SP600125 increased phosphorylation of PKC?II and tumor necrosis factor-??converting enzyme and induced their translocation into the plasma membrane. Finally, in vitro cell invasion experiments and bioinformatics analysis of data in The Cancer Genome Atlas breast cancer database revealed that JNK and PKC?II are important for Prss14/epithin-mediated cancer progression. These results provide important information regarding strategies against tumor metastasis. | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.subject | SERINE-PROTEASE | - |
dc.subject | PROSTATE-CANCER | - |
dc.subject | MATRIPTASE | - |
dc.subject | FILAMIN | - |
dc.subject | EPITHIN | - |
dc.subject | JNK | - |
dc.subject | CARCINOGENESIS | - |
dc.subject | SUPPRESSES | - |
dc.subject | ACTIVATION | - |
dc.subject | EXPRESSION | - |
dc.title | A JUN N-terminal kinase inhibitor induces ectodomain shedding of the cancer-associated membrane protease Prss14/epithin via protein kinase C?II | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Cho, Yongcheol | - |
dc.contributor.affiliatedAuthor | Kim, Chungho | - |
dc.identifier.doi | 10.1074/jbc.RA119.011206 | - |
dc.identifier.scopusid | 2-s2.0-85084795415 | - |
dc.identifier.wosid | 000536212600030 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.295, no.20, pp.7168 - 7177 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 295 | - |
dc.citation.number | 20 | - |
dc.citation.startPage | 7168 | - |
dc.citation.endPage | 7177 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | SERINE-PROTEASE | - |
dc.subject.keywordPlus | PROSTATE-CANCER | - |
dc.subject.keywordPlus | MATRIPTASE | - |
dc.subject.keywordPlus | FILAMIN | - |
dc.subject.keywordPlus | EPITHIN | - |
dc.subject.keywordPlus | JNK | - |
dc.subject.keywordPlus | CARCINOGENESIS | - |
dc.subject.keywordPlus | SUPPRESSES | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordAuthor | serine protease | - |
dc.subject.keywordAuthor | shedding | - |
dc.subject.keywordAuthor | PKC | - |
dc.subject.keywordAuthor | c-Jun N-terminal kinase (JNK) | - |
dc.subject.keywordAuthor | cell invasion | - |
dc.subject.keywordAuthor | breast cancer | - |
dc.subject.keywordAuthor | bioinformatics | - |
dc.subject.keywordAuthor | cell biology | - |
dc.subject.keywordAuthor | cell invasion | - |
dc.subject.keywordAuthor | ectodomain shedding | - |
dc.subject.keywordAuthor | JNK | - |
dc.subject.keywordAuthor | protein kinase C ? | - |
dc.subject.keywordAuthor | Prss14 | - |
dc.subject.keywordAuthor | epithin | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(02841) 서울특별시 성북구 안암로 14502-3290-1114
COPYRIGHT © 2021 Korea University. All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.