Three-dimensional interferometric scattering microscopy via remote focusing technique
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Il-buem | - |
dc.contributor.author | Moon, Hyeon-Min | - |
dc.contributor.author | Park, Jin-Sung | - |
dc.contributor.author | Zambochova, Katerina | - |
dc.contributor.author | Hong, Seok-Cheol | - |
dc.contributor.author | Cho, Minhaeng | - |
dc.date.accessioned | 2021-08-31T01:02:43Z | - |
dc.date.available | 2021-08-31T01:02:43Z | - |
dc.date.created | 2021-06-18 | - |
dc.date.issued | 2020-05-01 | - |
dc.identifier.issn | 0146-9592 | - |
dc.identifier.uri | https://scholar.korea.ac.kr/handle/2021.sw.korea/56059 | - |
dc.description.abstract | Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visualize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We anticipate that RF-iSCAT would enhance the utility of iSCAT for dynamics study. (C) 2020 Optical Society of America | - |
dc.language | English | - |
dc.language.iso | en | - |
dc.publisher | OPTICAL SOC AMER | - |
dc.subject | TRACKING | - |
dc.title | Three-dimensional interferometric scattering microscopy via remote focusing technique | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Hong, Seok-Cheol | - |
dc.contributor.affiliatedAuthor | Cho, Minhaeng | - |
dc.identifier.doi | 10.1364/OL.386172 | - |
dc.identifier.scopusid | 2-s2.0-85084501291 | - |
dc.identifier.wosid | 000535919200041 | - |
dc.identifier.bibliographicCitation | OPTICS LETTERS, v.45, no.9, pp.2628 - 2631 | - |
dc.relation.isPartOf | OPTICS LETTERS | - |
dc.citation.title | OPTICS LETTERS | - |
dc.citation.volume | 45 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2628 | - |
dc.citation.endPage | 2631 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Optics | - |
dc.relation.journalWebOfScienceCategory | Optics | - |
dc.subject.keywordPlus | TRACKING | - |
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